新型海藻酸盐水凝胶真皮支架材料的制备及其表征  

Preparation and exosyndrome of sodium alginate hydrogel as a dermal tissue engineering scaffold

作　　者：朱家源[1] 王琴梅[1] 朱斌[1] 唐冰[1] 蔡浩[1] 

机构地区：[1]中山大学附属第一医院烧伤外科,广东省广州市510080

出　　处：《中国组织工程研究与临床康复》2008年第41期8099-8102,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:海藻酸钠水凝胶具有与真皮基质成分蛋白多糖类似的结构,是较为理想的成纤维细胞培养材料,但以往制备海藻酸钠水凝胶大部分都是以CaCl2为交联剂,这一体系存在严重不足。目的:利用碳化二亚胺作为催化剂、乙二胺作为交联剂制备新型海藻酸盐水凝胶支架材料,并对其性能进行表征,探讨其作为组织工程支架材料的可行性。设计、时间及地点:形态学描述及自身对照实验,于2005-10/2006-10在中山大学附属第一医院骨科实验室完成。材料:自制乙二胺溶液,碳化二亚胺和海藻酸钠购自Sigma公司。方法:利用碳化二亚胺作为催化剂、乙二胺作为交联剂,采用共价交联及冻干法制备新型海藻酸盐水凝胶支架,对照为用传统交联剂CaCl2制备的样品。主要观察指标:观察海藻酸钠的理想反应浓度;用扫描电镜观察藻酸盐组织工程支架的微观结构,排液法测定其孔隙率及含水量;并观察其体外降解规律。结果:成功制备了海藻酸盐水凝胶真皮支架,该支架材料的海藻酸钠理想反应浓度为2%,其含水量为(95.32±1.24)%,孔隙率为(85.55±0.98)%,均高于对照材料;2周后海藻酸盐水凝胶的体外降解率为(12.26±2.25)%。结论:新型海藻酸盐组织工程真皮支架克服了传统以钙离子作为交联剂的诸多缺点,其吸水性、孔隙率、降解性能满足真皮支架材料的物理性能所需,能够做为成纤维细胞的三维培养系统。BACKGROUND： Sodium alginate hydrogels are identical with the proteoglycan, a dermal matrix component, regarding the structure. It is an ideal material for fibroblast culture. However, Sodium alginate hydrogels are prepared taking CaCl2 as cross linking agent, which is a defective method. OBJECTIVE： Taking 1-ethyl-3-（3-dimethylaminopropyl）-carbodiimide as the catalyst and ethylene diamine as cross linking agent, the sodium alginate hydrogels are prepared as a new kind of tissue engineering scaffold, its physical chemistry performance is analyzed, and its feasibility to be dermis substitute as the tissue engineering scaffold is approached. DESIGN, TIME AND SETTING： The experiment, a morphology observation and self-controlled study, was performed in Department of Orthopedics, the First Affiliated Hospital of Sun Yat-sen University from October 2005 to October 2006. MATERIALS： The 1-ethyl-3-（3-dimethylaminopropyl）-carbodiimide, ethylene diamine and sodium alginate were purchased from sigma Company. METHODS： Using 1-ethyl-3-（3-dimethylaminopropyl）-carbodiimide as a catalyst and ethylene diamine as a cross linking agent, sodium alginate hydrogel scaffolds were prepared with covalent cross-linking, freezing and drying method. Conventional sample prepared using CaCl2 as cross linking agent was taken as controls. MAIN OUTCOME MEASURES： The ideal response concentration of sodium alginate was observed; the microstructure of alginate scaffold for tissue engineering was detected using scanning electron microscope; the water content and porosity of alginate scaffold were determined using pushing liquid aside; the degradation in vitro was also observed. RESULTS： The sodium alginate hydrogels for tissue engineering dermis were prepared successfully. Ideal reaction thickness was 2%, porosity was （85.55±0.98）%, the water content was （95.32±1.24）%, which were higher than those of control materials; The sodium alginate hydrogel lost （12.26±2.25）% of its weight in 2 weeks. CONCLUSION： Compar

关 键 词：海藻酸盐 水凝胶 支架 组织工程真皮 交联剂 乙二胺 生物材料 

分 类 号：R318[医药卫生—生物医学工程]