非程序DNA合成法检测水有机提取物的致突变性研究  

The Detection of Mutagenicity of Water Organic Extract Substances by UDS-test

作　　者：韩发彬[1] 王福琳[1] 刘春生[1] 袁晓艳 丁恬[1] 

机构地区：[1]山东医科大学环境卫生学教研室

出　　处：《环境与健康杂志》1990年第4期152-154,共3页Journal of Environment and Health

摘　　要：非程序DNA合成试验已被用作检测化学物质的致癌性/诱变性,筛选化学致癌物的一项重要参考指标。本文用人外周血淋巴细胞的UDS试验对受污染河水及其沿岸饮用井水有机提取物的致突变性进行了检测。结果表明河水及井水有机提取物均可诱发CPM值的明显增加,用不同浓度按等比级数剂量进行试呈明显的剂量反应关系,提示该河水与井水有机提取物均有直接损伤DNA的能力,从而诱发突变。Unshedule DNA synthesis test is a very important indicator which is used to detect the mutagenesis and carcinogenesis of chemical carcinogens. Here we used the UDS test of human beings lymphocytes to detect the mutagenesis of organic extract substances ( OES ) from polluted river water and drinking well water of residents living along the river. The rssults showed that both OES of river water and well watsr can increase the CPM of lymphocyte apparently and there is a significant dose-response relationship. This indicate both river water and well water can destroy DNA of lymphocyte.

关 键 词：DNA合成法 检测 水 有机物 

分 类 号：X832.02[环境科学与工程—环境工程]