不同种类自体骨髓干细胞移植对梗死心肌左室重构的效果比较(英文)  

Improvement of left ventricle remodeling by transplanting various autologous bone marrow stem cells

作　　者：李树仁[1] 齐晓勇[1] 张建清[1] 王天红[1] 党懿[1] 孟寸良[1] 刘会良[1] 李英肖[1] 胡福莉[1] 吴迪[1] 董洁[1] 荀丽颖[1] 郜利会[1] 靳福昌[1] 

机构地区：[1]河北省人民医院心脏中心,河北省石家庄市050051

出　　处：《中国组织工程研究与临床康复》2008年第47期9371-9377,共7页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:骨髓干细胞移植可改善心功能、预防心室重构,目前用于移植的成体骨髓干细胞有骨髓单个核细胞、骨髓间充质干细胞和内皮祖细胞等,不同种类的骨髓干细胞移植后的效果及具体机制尚不清楚。目的:比较自体骨髓单个核细胞、骨髓间充质干细胞冠状动脉移植对急性心肌梗死后心室重构的影响。设计、时间及地点:随机对照动物实验,于2005-03/2006-12在河北省人民医院临床研究中心、河北医科大学电镜室及大连宝生物进行。(basic fibroblast growth factor,bFGF)材料:36只冀中白猪随机分为4组:假手术组6只、模型组10只、骨髓单个核细胞组10只、骨髓间充质干细胞组10只。方法:采用梯度密度离心法分离培养猪自体骨髓单个核细胞,以贴壁法分离培养猪自体骨髓间充质干细胞,移植前均行胶体金标记。除假手术组外,其余3组均以球囊导管压迫冠状动脉前降支的方法建立猪急性心肌梗死模型。造模后90min,经导管由冠状动脉腔内骨髓单个核细胞组移植自体骨髓单个核细胞(6.0±1.3)×107个,骨髓间充质干细胞组移植自体骨髓间充质干细胞(4.5±2.1)×107个,培养28d。主要观察指标:光镜及电镜观察心肌组织病理学改变;超声检查心功能变化;免疫组织化学检测心肌血管数、心肌细胞凋亡、心肌组织核因子κB及肌钙蛋白I阳性表达情况,及其与心功能的关系;RT-PCR法检测心肌血管内皮生长因子(vascular endothelial growth factor,VEGF)、碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)mRNA的表达,及其与心功能的关系。结果:①骨髓间充质干细胞组在梗死区、梗死边缘区均可见大量血管增生,冠脉血管周围可见异常细胞团生长,骨髓单个核细胞组有较多的毛细血管"芽生"现象。②细胞移植前各组心功能指标基本相似(F=1.550,P>0.05)。移植后28d与模型组比较,其余3组左心室射血分数均明显降低(FBACKGROUND： Bone marrow stem cell transplantation can improve heart function and prevent ventricle remodeling. At present, the adult bone marrow stem cells used for transplantation primarily included bone marrow mononuclear cells （BM-MNCs） and mesenchymal stem cells （MSCs）, and endothelial progenitor cells. The curative effects and precise mechanisms of transplantation of various bone marrow stem cells remain unknown. OBJECTIVE： To compare the effects of transplantation of autologous BM-MNCs and MSCs via the coronary artery on ventricle remodeling subsequent to acute myocardial infarction （AMI）. DESIGN, TIME AND SETTING： Randomized controlled animal experiment performed at the Center for Clinical Research, Hebei Provincial People＇s Hospital, Electron Microscope Room,Hebei Medical University between March 2005 and December 2006. MATERIALS： Thirty-six male Jizhong pigs, were randomly divided into 4 groups： control group （n = 6）, infarct model group （n = 10）, BM-MNC group （n = 10）, and MSC group （n = 10）. METHODS： Porcine autologous BM-MNCs were isolated by gradient density centrifugation, and MSCs were obtained by adherence method. Prior to transplantation, both BM-MNCs and MSCs were colloidal gold labeled. Except the infract model group, pigs in the other 3 groups were developed into AMI models by oppressing the left anterior descending branch with balloon catheter. Ninety minutes after modeling, （6.0± 1.3）× 10^7 autologous B M-MNCs and （4.5±2.1）×10^7 MSCs were respectively transplanted into pigs in the BM-MNC group and the MSC group via the coronary artery and cultured for 28 days. MAIN OUTCOME MEASURES： Observation of pathological changes of cardiac muscle tissue by light and electron microscope; Examination of cardiac function by ultrasonograph; Detection of the number of blood vessels and apoptotic myocardial cells, and expression of nuclear factor- κB （NF- κB） and troponin I and its correlation to cardiac function by immunohistochemistry; Detection

关 键 词：骨髓单个核细胞 骨髓间充质干细胞 移植 心肌梗塞 心室重构 

分 类 号：R394.2[医药卫生—医学遗传学]