白细胞介素-1通过JNK信号通路调控Schlafen2基因的表达  

Interleukin-1 Regulates Schlafen2 Gene Expression Through JNK Signal Pathway

作　　者：胡迎春[1] 方玲[2] 周乔丹[3] 陈忠民[1] 李刚[1] 吴德昌[1] 霍艳英[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850 [2]兰州大学基础医学院病理生理教研室,甘肃兰州730000 [3]重庆医科大学病理学教研室,重庆400016

出　　处：《生物技术通讯》2008年第6期803-806,共4页Letters in Biotechnology

基　　金：国家自然科学基金(30471946);北京市自然科学基金(7052056)

摘　　要：目的:在多效生长因子(Ptn)基因稳定沉默的小鼠胚胎成纤维细胞Ptn-siRNA B/MEF241中,研究白细胞介素-1(IL-1)调控Schlafen2(Slfn2)基因表达的机制。方法:应用Northernblot检测Ptn沉默细胞Ptn-siRNAB/MEF241处于不同生长密度时Slfn2基因的表达变化,以确定Ptn沉默细胞中Slfn2基因的表达是否受到某种分泌性细胞因子的调控;用不同浓度的IL-1α中和抗体及IL-1受体拮抗剂处理Ptn沉默细胞,通过Northern blot检测细胞内Slfn2表达的抑制情况;用不同浓度的IL-1α中和抗体及IL-1受体拮抗剂处理Ptn沉默细胞不同时间,通过Western blot检测细胞中JNK磷酸化水平;Northern blot检测SP600125(JNK/MAPK通路抑制剂)对Ptn沉默细胞中Slfn2基因表达的影响。结果:Ptn沉默细胞中Slfn2基因的表达水平同细胞密度相关;用中和抗体和受体拮抗剂阻断IL-1通路,Slfn2表达受到显著抑制;IL-1受到抑制会影响JNK通路的活化;阻断JNK通路,Slfn2的表达受到显著抑制。结论:IL-1可以通过JNK通路诱导Slfn2的表达。Objective： To investigate the mechanisms of interleukin-1（IL-1） involved in expression regulation of Schlafen2 （Slfn2） in the pleiotrophin（Ptn） knock down mouse embryo fibroblast cells line. Methods： The effect of cell density of Ptn- siRNA B/MEF241 cells on Slfn2 expression was examined through Northern blot. Different concentration of IL-1α affinity purified polyclonal antibody and rhlL-lra were used to incubate Ptn-siRNA B/MEF241 cells and the expression of Slfn2 gene was investigated. Different concentration of IL-lct antibody and rhlL-lra were used to incubate Ptn-siRNA B/ MEF241 cells and the phosphorylation level of JNK was examined through Western blot. The effect of SP600125（JNK/ MAPK inhibitor） on transcription of Slfn2 in Ptn-siRNA B/MEF241 cells was investigated through Northern blot. Results： Slfn2 expression was subject to cell density control. Interruption of IL-1 could suppress Slfn2 gene expression. Suppression of IL-1 could affect activation of JNK. Interruption of JNK could suppress Slfn2 gene expression. Conclusion： The expres- sion of Slfn2 was regulated by IL-1 through the JNK/MAPK pathway in Ptn-siRNA B/MEF241 cells.

关 键 词：多效生长因子 白细胞介素-1 JNK通路 Schlafen2基因 基因表达 

分 类 号：Q25[生物学—细胞生物学] Q78