一种快速判定细胞培养中支原体污染的方法——瓜氨酸测定法  

A Rapid Detection of Mycoplasma Contamination in Cell Culture Using Citrulline Assay

作　　者：梁少东[1] 黄小乐[1] 严媛[1] 杨琴[1] 肖成祖[1] 

机构地区：[1]广州铭康生物工程有限公司,广东广州510730

出　　处：《生物技术通讯》2008年第6期892-894,共3页Letters in Biotechnology

摘　　要：目的:开发一种简便、快速、能及时发现细胞培养中支原体污染的方法。方法:用HPLC检测细胞培养中瓜氨酸是否存在及其量的大小。结果:当细胞培养被支原体污染时,培养基中精氨酸量明显下降,同时有瓜氨酸出现;当支原体被消除后,瓜氨酸即消失。结论:在细胞培养中瓜氨酸的出现与支原体污染的关系是特异的,用HPLC在2h内即可检出,表明该方法可靠、简便、快速,可作为细胞培养过程中支原体污染的常规监测手段。Objective： To develop a simple and rapid method for detecting mycoplasmas contamination in cell culture. Methods： Measuring the content of citrulline in cell cultures by HPLC. Results： When the cell culture was contaminated by mycoplasma, the amount of arginine decreased obviously and the citrulline appeared in cell culture; when the my- coplasma was eliminated, the citrulline was disappeared. Conclusion： The appearance of citrulline in cell culture is particularly relative with mycoplasma contamination, and it can detected in 2 h by HPLC. It is a reliable, simple and rapid way as a regular inspection for mycoplasma contamination.

关 键 词：细胞培养 支原体污染 瓜氨酸 精氨酸脱亚氨酶 高效液相色谱 

分 类 号：Q813[生物学—生物工程] Q503