半滑舌鳎和塞内加尔鳎养殖群体遗传变异的RAPD分析  

RAPD Analysis of Genetic Variability in Two Cultured Stocks of Cynoglossus semilaevis and Solea senegalensis

作　　者：杨奔[1,2] 尤锋[1] 李军[1] 吴志昊[1] 王波[3] 徐冬冬[1] 于道德[1] 倪静[1] 徐世宏[1] 徐永立[1] 张培军[1] 

机构地区：[1]中国科学院海洋研究所,山东青岛266071 [2]哈尔滨工业大学(威海),山东威海264209 [3]国家海洋局第一海洋研究所, 山东青岛266061

出　　处：《海洋科学进展》2008年第4期506-511,共6页Advances in Marine Science

基　　金：国家高技术研究发展计划探索课题--鲆鲽类高产、抗逆品种的培育(2006AA10A404);国家自然科学基金项目--外源因子影响鲆鲽鱼类性别决定形成的研究(30571445)

摘　　要：采用RAPD方法对半滑舌鳎和塞内加尔鳎的养殖群体进行遗传变异分析。从78条随机引物中筛选出20条用于群体遗传学分析,2个群体共扩增出260条DNA片段,其中半滑舌鳎187条,塞内加尔鳎180条,片段大小为200-2 000 bp。半滑舌鳎和塞内加尔鳎养殖群体的多态片段比例分别为63.10%和60.56%,Nei的多样性指数和Shannon的信息指数分别为0.266 4和0.237 5,0.385 2和0.349 8。与其它鲆鲽鱼类养殖群体相比,半滑舌鳎和塞内加尔鳎养殖群体的遗传多样性水平略低;2种鱼群体间的遗传相似性系数和遗传距离分别为0.458 3和0.723 1,它们的遗传变异主要来自群体内。The RAPD technology was used to analyze the genetic variation of cultured stocks of Cynoglossus semilaevis and Solea senegalensis. Out of 78 random oligonucleotide primers, 20 primers were selected for stock genetics analysis. Total 260 bands were amplified, including 187 of Cynoglossus semilaevis and 180 of Solea senegalensis, ranging between 200 and 2 000 bp. The percentage values of polymorphic bands of these two stocks were respectively 63.10% and 60.56%, and their Nei＇s genetic diversity values were 0. 266 4 and 0. 237 5, respectively, while their Shannon indexes were 0. 385 2 and 0. 349 8. The results suggested thatthe genetic variabilities of these two cultured stocks were a little lower than those of cultured stocks of other flatfish. The genetic similarity and genetic distance between these two cultured stocks were 0. 458 3 and 0. 723 1, respectively. And the most genetic variabiltiy take place in the corresponding stock.

关 键 词：半滑舌鳎 塞内加尔鳎 养殖群体 RAPD 遗传变异 

分 类 号：Q347[生物学—遗传学]