微泡介导下诊断超声波开放人血脑屏障的可行性研究:体外微泡破坏实验  

The feasibility study of diagnostic ultrasound in presence of microbubbles for disruption of blood bram-barrier:microbubbles destruction experiment in vitro

作　　者：付赤学[1] 谭开彬[1] 高云华[1] 刘政[1] 赵洋[1] 高顺记[1] 黄嘉[1] 

机构地区：[1]第三军医大学新桥医院超声科,重庆市400037

出　　处：《临床超声医学杂志》2008年第11期721-724,共4页Journal of Clinical Ultrasound in Medicine

基　　金：国家自然科学基金项目(30672413)

摘　　要：目的探讨诊断超声经人颅骨破坏微泡的可行性及条件,为下一步开展诊断超声联合微泡开放人血脑屏障的在体实验研究奠定基础。方法建立体外微泡破坏实验装置,分别考察GE Vivid 7、Sequoia 512和Philips iE 33超声仪器对儿童、青年和老年颅骨的颞骨窗、枕骨窗和顶骨窗5.0 cm处微泡的破坏情况。选择Sequoia 512超声仪器以最低频率(1.75 MHz)、机械指数(1.9)经青年颞骨辐照微泡后,考察不同辐照深度微泡的破坏情况。结果3种超声诊断仪经所有颅骨的颞骨窗、枕骨窗辐照后后都能明显地破坏微泡,但经顶骨不能击破微泡。Sequoia 512超声仪器经青年颞骨后在辐照深度7.5 cm处仍能明显地破坏微泡。结论诊断超声波经人颅骨颞骨窗、枕骨窗仍能破坏微泡,具有联合微泡开放人血脑屏障的潜在可能性。Objective To explore the feasibility of mierobubbles destruction with diagnostic ultrasound irradiation through the human cranial bone, and to investigate the condition for further disruption of the blood brain barrier in vivo with diagnostic ultrasound and microbubbles. Methods Microbubbles destruction experimental facility was established in vitro. The GE Vivid 7, Sequoia 512 and Philips iE 33 ultrasound equipments were applied for destruction of microbubbles through different parts of skulls（os temporale , occipital bone , hregmatic bone）with different ages at the depth of 5.0 cm. Sequoia 512 irradiation of themierobubblcs with the lowest frequency（ 1.75 MHz） and maximum mechanical index through the young os temporale was performed to analyze the microbubbles destruction in different irradiation depth. Results The microbubbles were destructed conspicuously by three types of diasonographies exposure through the puerile, young and senile os temporale and occipital bone except through bregmatic bone.At the depth of 7.5 cm, Sequoia 512 diasonography irradiation could still significantly destruct the microbubbles. Conclusion Diagnostic ultrasound associated with microbubbles might open the blood- brain barrier.

关 键 词：超声检查 诊断 微泡 血脑屏障 

分 类 号：R445.1[医药卫生—影像医学与核医学] R651.15[医药卫生—诊断学]