11个绵羊品种MSTN基因非翻译区的变异  

Variation of MSTN gene UTR in eleven sheep breeds

作　　者：孟详人[1,2] 郭军[1] 赵倩君[1] 马月辉[1] 关伟军[1] 刘娣 狄冉[1] 乔海云[1,3] 那日苏[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]黑龙江省农业科学院畜牧研究所,哈尔滨150086 [3]河北工程大学农学院,邯郸056000

出　　处：《遗传》2008年第12期1585-1590,共6页Hereditas（Beijing)

基　　金：“十一五”国家科技支撑项目(编号:2006BAD13B08);国家自然科技资源平台项目(编号:2005DKA21101);“十一五”863计划项目(编号:2006AA10Z198)资助~~

摘　　要：利用PCR-RFLP技术对特克塞尔羊、夏洛莱羊、小尾寒羊、蒙古羊、乌珠穆沁羊、阿勒泰羊、呼伦贝尔羊、塔什库尔干羊、多浪羊、湖羊和岗巴羊11个品种的345个个体的肌肉生长抑制素(Myostatin,MSTN)基因非翻译区(UTR)的变异进行了多态性分析。结果表明大小为271bp和1003bp的扩增片段经限制性内切酶MboⅡ和BsaⅠ酶切后表现多态,经卡方检验所有品种在该基因座位均处于平衡状态(P>0.05),3种基因型在11个绵羊品种中的分布差异极显著(P<0.01)。通过限制性内切酶HpyCH4Ⅳ酶切实验,证明我国9个地方绵羊品种不存在特克塞尔绵羊中发现的导致肌肉发达的SNP位点,并在3′UTR区发现了个别碱基突变位点能够形成miRNA作用的靶基序,测序表明3′UTR区的突变频率较高。Abstract： PCR-RFLP was applied to analyze the polymorphism of MSTN gene UTR in 345 sheep that comprised of eleven sheep breeds, namely Texel sheep, Charolais sheep, Small-tailed Han sheep, Monggolian sheep, Ujumqin sheep, Altay Fat-rumped sheep, Hulunbeir sheep, Tashikurgan sheep, Duolang sheep, Hu sheep, and Gangba sheep. A 271 bp and a 1 003 bp long PCR products were digested with Mbo !1 and Bsa I to demonstrate polymorphism in the eleven sheep breeds, which were all at Hardy-Weinberg equilibrium （P〉0.05）. The distribution of 3 genotypes in 11 sheep breeds was signifi- cantly different （P〈0.01）. Digestion of the PCR products with HpyCH41V proved that 9 domestic local sheep breeds were different from Texel sheep in the SNP site that was associated with muscularity. The individual mutation base could gener- ate the motifs for miRNA in the 3＇UTR, and sequencing analysis demonstrated high frequency of mutation in the 3＇UTR region.

关 键 词：MSTN UTR PCR—RFLP MicroRNA 多态性 

分 类 号：S826[农业科学—畜牧学]