9-顺式维甲酸抑制佛波酯诱导人单核细胞系THP-1向巨噬细胞分化  

9-cis Retinoid Acid Inhibits the Differentiation of THP-1 Human Monocyte Cell Line into Macrophage Induced by Phorbol-12-Myristate-13-Acetate

作　　者：周磊[1] 何奔[1] 沈玲红[1] 曾锦章[2] 胡刘华[1] 卜军[1] 王力[2] 

机构地区：[1]上海交通大学医学院附属仁济医院心内科,上海市200127 [2]中国科学院上海生命科学研究所,上海市200233

出　　处：《中国动脉硬化杂志》2008年第9期673-676,共4页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金(30670880和30600242);上海市科委基础研究重点项目(05JC14037和08XD1402600)

摘　　要：目的探讨视黄醇类X受体特异性激动剂9-顺式维甲酸对佛波酯诱导人单核细胞系THP-1向巨噬细胞分化的影响。方法体外培养人单核细胞系THP-1,经佛波酯诱导分化为巨噬细胞,同时给予9-顺式维甲酸对佛波酯诱导分化进行干预,通过相差显微镜观察细胞形态,MTT比色法检测细胞贴壁率,流式细胞仪检测与单核/巨噬细胞分化有关的细胞表面标志物CD11b和CD36的表达。结果9-顺式维甲酸干预后梭形、分支状细胞明显减少,与佛波酯单独作用组相比细胞贴壁率减少50%(P<0.01),细胞表面标志物CD11b和CD36分别减少50%和28%(P<0.01)。结论视黄醇类X受体特异性激动剂9-顺式维甲酸可明显抑制佛波酯诱导人单核细胞系THP-1向巨噬细胞分化。Aim To investigate the effect of 9-cis-retinoid acid （9-cisRA）, a specific agonist of retinoid X receptors （RXR） on the differentiation of THP-1 human monocyte cell induced by phorbol-12-myristate-13-acetate （PMA）. Methods Human monocyte cell line THP-1 was cultured and induced to macrophage by PMA treatment. The effect of 9-cisRA on the differentiation induced by PMA was studied. Cell morphology was observed by phase contrast microscope and the adhesion rate of THP-1 was detected by methyl thiazolyltetrazolium （MTT） assay. The cell surface markers involved in differentiation of monocyte/macrophage （CD11b, CD36） were analyzed by flow cytometry （FACS）. Results The metamorphotic cells were decreased in 9-cisRA group. THP-1 cell adhesion rate was inhibited by 50% （P〈0.01）, and the cell surface markers （CD11b, CD36） were decreased by 50% and 28% （P〈0.01） in 9-cisRA treated group. Conclusion The specific agonist of RXR, 9-cisRA could effectively inhibit the differentiation of THP-1 human monocyte induced by PMA.

关 键 词：内科学 视黄醇类X受体 单核细胞 巨噬细胞 动脉粥样硬化 细胞分化 

分 类 号：R5[医药卫生—内科学]