钩藤碱和异钩藤碱对血管紧张素Ⅱ致血管平滑肌细胞凋亡的影响及其机制  

Effects of Rhynchophylline and Isorhynchophylline on the Apoptosis of Vascular Smooth Muscle Cell Induced by Angiotensin Ⅱin Rats

作　　者：李运伦[1] 

机构地区：[1]山东中医药大学附属医院心脏内科,山东省济南市250011

出　　处：《中国动脉硬化杂志》2008年第9期681-684,共4页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金资助课题(30300466);山东省自然科学基金资助课题(Y2006C99);山东省卫生系统高层次人才1020工程资助课题

摘　　要：目的探讨钩藤碱和异钩藤碱对血管紧张素Ⅱ致血管平滑肌细胞凋亡的影响及其机制。方法建立血管紧张素Ⅱ诱导血管平滑肌细胞凋亡模型,通过倒置相差显微镜、激光共聚焦显微镜、流式细胞术和逆转录聚合酶链反应观察钩藤碱和异钩藤碱对细胞形态、细胞凋亡率、细胞[Ca2+]i浓度、Bcl-2和Bax蛋白表达与mRNA转录的影响。结果钩藤碱和异钩藤碱能诱导血管平滑肌细胞凋亡,提高细胞凋亡百分率,降低细胞[Ca2+]i浓度,下调Bcl-2蛋白表达、上调Bax蛋白表达,升高BaxmRNA/Bcl-2mRNA的比值。结论钩藤碱和异钩藤碱具有诱导血管平滑肌细胞凋亡的作用,其机制与降低细胞[Ca2+]i浓度、下调Bcl-2蛋白表达及mRNA转录、上调Bax蛋白表达及mRNA转录有关。Aim To study the effect of rhynchophylline and isorhynchophylline on the apoptosis of vascular smooth muscle cell （VSMC） induced by angiotensin Ⅱ （AngⅡ） its mechanism in rats. Methods The apoptosis model in VSMCs induced by angiotensin Ⅱ was established. The effect of rhynchophylline and isorhynchophylline on the cell morphology, apoptosis rate, [Ca2＋]i concentration, Bcl-2 and Bax protein expression and mRNA expression were observed by invert phase contrast microscope, flow cytometry and RT-PCR. Results The rhynchophylline and isorhynchophylline could induce the apoptosis of VSMCs, increase cell apoptosis rate, decrease cell [Ca2＋]i concentration and down regulate Bcl-2, Bax protein and mRNA expression. Conclusion The rhynchophylline and isorhynchophylline may induce the apoptosis of VSMC, whose mechanisms might be related to decreasing cell [Ca2＋]i concentration and down regulating Bcl-2 and Bax protein expression and mRNA expression.

关 键 词：中药学 钩藤碱 异钩藤碱 血管平滑肌细胞 细胞凋亡 信号转导 血管紧张素Ⅱ 

分 类 号：R28[医药卫生—中药学]