2型糖尿病大鼠动脉粥样硬化模型不同制备方法探讨  

Evaluation of the Methods for Establishing Type 2 Diabetic Atherosclerotic Rat Models

作　　者：徐雷[1] 冯波[1] 王华[1] 王军臣[2] 符雪莲[2] 

机构地区：[1]同济大学附属东方医院内分泌科,上海市200120 [2]同济大学附属东方医院病理科,上海市200120

出　　处：《中国动脉硬化杂志》2008年第9期707-709,共3页Chinese Journal of Arteriosclerosis

基　　金：上海市卫生局科技发展基金(054087)

摘　　要：目的比较和探索制备2型糖尿病动脉粥样硬化大鼠模型的不同方法。方法健康雄性Wistar大鼠5只作为对照组,雄性自发性糖尿病模型GK大鼠随机分为高甘油三酯组(12周)、高胆固醇12周组和高胆固醇24周组,每组5只。组织病理学观察各组动脉粥样硬化形成情况。结果四组大鼠血管各层结构基本正常,未见典型动脉粥样硬化病变。免疫组织化学染色显示,四组主动脉壁均无巨噬细胞浸润,中膜及内膜层平滑肌细胞排列规则。结论对于2型糖尿病大鼠动脉粥样硬化模型的制备,这三种方法在Wistar大鼠均不易复制成功。Aim To compare and investigate the different methods for establishing type 2 diabetic atherosclerosis rat models. Methods 5 Wistar rats were chosen control group and 15 GK rats were randomly divided into three groups： hypertriglyceride diet （12 weeks） group, hypercholesterol diet with vitamin D （12 weeks） group and hyperchlesterol diet with vitamin D （24 weeks） group. Histopathology was used to observe the atherosclerotic lesions. Results There were no obvious morphologic changes in these four groups by light microscope. The macrophage-related antigen expressions in the aorta were not observed in all the four groups. Absence in abnormal expression of SMC-related antigens was shown in all of them. Conclusion All of the three methods were not successful in establishing diabetic atherosclerotic rat models in Wistar rats.

关 键 词：内科学 大鼠 动物模型 糖尿病 动脉粥样硬化 

分 类 号：R5[医药卫生—内科学]