广东省2007年度非伤寒沙门菌监测及病原学特征分析  

Surveillance and pathogenic analysis on non-typhoidal Salmonella in Guangdong province, 2007

作　　者：柯碧霞[1] 邓小玲[1] 张丽华[2] 陈嘉琳[3] 柯昌文[1] 郭川[4] 曹海燕[5] 林卫[6] 谭海玲[1] 李柏生[1] 

机构地区：[1]广东省疾病预防控制中心,广州510300 [2]东莞东华医院 [3]湛江市疾病预防控制中心 [4]汕头市疾病预防控制中心 [5]惠州市中心医院 [6]韶关市始兴县疾病预防控制中心

出　　处：《中华流行病学杂志》2008年第12期1199-1203,共5页Chinese Journal of Epidemiology

基　　金：全球沙门菌监测网资助项目（GSS）;中美新发和再发传染病合作计划资助项目（1U2GGH000018-01）;感谢湛江市、汕头市、始兴市、南雄市、东莞市、惠州市以及中国疾病预防控制中心,东莞东华医院、广州医学院第二附属医院、汕头医学院第一附属医院、广东医学院第一附属医院、新海医院、惠州市中心医院、南雄市中医院、始兴县城郊医院以及阚飙和冉陆老师对本研究的大力支持与帮助.

摘　　要：目的了解广东省腹泻病患者中非伤寒沙门菌感染情况和菌株的血清型别、分布、耐药性变化。方法对纳入研究的腹泻病患者进行非伤寒沙门菌检测，对分离到的菌株进行血清分型、药物敏感性试验和脉冲场凝胶电泳（PFGE）分型。结果2007年度共检测1128份腹泻粪便标本，分离到71株沙门菌，阳性检出率为6．29％；共分得29种血清型，肠炎和鼠伤寒沙门菌居多；大多数沙门菌对常用的头孢类和喹诺酮类抗生素敏感，鼠伤寒沙门菌的耐药率普遍较肠炎和斯坦利沙门菌高；除肠炎沙门菌外，其余的血清型没有同一PFGE型别的菌株；用XbaⅠ酶切17株肠炎沙门菌可分为PFGE—XbaⅠ1～8型，其中PFGE—XbaⅠ4型为优势型别。用SfiⅠ和NotⅠ酶对12株肠炎沙门菌进行再分型，综合用XbaⅠ／SfiⅠ／NotⅠ三种酶的结果发现仍有三组菌的PFGE图谱是完全一致的。结论2007年度广东省非伤寒沙门菌的感染多数为散发病例，头孢类和喹诺酮类抗生素是治疗非伤寒沙门菌感染的首选药物。Objective To understand non-typhoid Salmonella in diarrhea patients from Guangdong province in order to timely discover the outbreaks caused by them as well as to grasp the serotypes, antibiotic resistance and pulse-field gel eleetrophoresis （PFGE） types of those strains isolated from this surveillance program. Methods Salmonella strains from diarrhea patients were detected and all the positive strains were tested by serum agglutination, antibiotic susceptibility and PFGE. Results 71 non- typhoid Salmonella strains were isolated from 1128 stoop samples, with a positive rate of 6.29 %. All the strains were divided into 29 serotypes, with Salmonella serotype enteritidis and typhimurium showing the most common serotypes. Most of the strains were sensitive to cephalosporins and quinolones. The antibiotic resistance rates of S. typhimurium were higher than S.enteritidis and S.stanley. Other than S. enteritidis, all the serotype strains did not have the same type of PFGE. 17 S. enteritidis strains digested by Xba Ⅰ were divided to 8 PFGE types while the PFGE 4 type appeared the most common one. 12 S. enteritidis strains were typed again by Sfi Ⅰ and Not Ⅰ , and there were still 3 groups of strains showing the same PFGE pattern. Conclusion Most of the infection caused by non-typhoidal Salmonella was sporadic in Guangdong province in 2007. Cephalosporins and quinolones seemed the best in curing the infection of non-typhoidal Salmonella at the clinics.

关 键 词：非伤寒沙门菌 病原学特征 监测 

分 类 号：R181.3[医药卫生—流行病学]