α-酮戊二酸对仔猪小肠组织学形态与功能的影响  

Effects of α-ketoglutarate on Histological Morphology and Function of Small Intestine in Piglets

作　　者：胡泉舟[1] 侯永清[1] 丁斌鹰[1] 朱惠玲[1] 刘玉兰[1] 王猛[1] 肖华丽[1] 

机构地区：[1]武汉工业学院动物营养与饲料科学湖北省重点实验室,武汉工业学院饲料科学系,武汉430023

出　　处：《动物营养学报》2008年第6期662-667,共6页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：湖北省自然科学基金计划创新群体项目(2007ABC009);国家“十一五”科技支撑计划(2006BAD12B07)

摘　　要：本研究通过试验探讨不同水平的α-酮戊二酸(AKG)对小肠组织学形态和功能的影响。试验1和试验2各选取32头仔猪,按体重随机分为4个处理,分别饲喂添加0、0.5%、1.0%和2.0%AKG的试验日粮;于试验第14天,测定血浆D-木糖浓度、二胺氧化酶(DAO)活性、内毒素含量。试验3选用12头仔猪,按体重随机分为2个处理,分别为基础日粮组和添加1.0%AKG的试验日粮组;于试验第14天,观察小肠黏膜形态。试验结果表明:试验1和试验2中,D-木糖含量均随AKG水平的增高而上升(P<0.05),当AKG水平为1.0%时达到最高(二次,P<0.05);试验1中血浆中的DAO活性随着AKG水平增高而降低(P<0.01),当AKG水平为1.0%时最低(二次,P<0.05);试验3中1.0%AKG组各肠段绒毛高度与隐窝深度比值(VH/CD)较对照组显著下降(P<0.05)。由试验结果得出,日粮中添加AKG可在一定程度上改善仔猪的小肠组织学形态和吸收功能,以1.0%比例添加效果较好。This research was conducted to evaluate the effects of different levels of dietary α-ketoglutarate （AKG） on histological morphology and function of small intestine in piglets. Both the experiment I and experiment 2 included 32 piglets, and they were randomly divided into 4 treatments on the basis of weight with supplementary AKG levels of 0, 0.5%, 1.0% and 2.0% respectively in the diet. On the 14th day of the experiment, the concentration of D-xylose and activity of diamine oxidase （DAO） in plasma and the content of endotoxin were studied. In the experiment 3, twelve piglets were randomly divided into 2 treatments on the basis of weight, and in the two treatments one group was fed with the basic diet and the other was fed with AKG supplement level of 1.0%. On the 14^th day, all pigs were weighed and sacrificed to observe the mucosa morphology. The small intestine was cut into three 5 cm segments at distal duodenum, mid-jejunum and mid-ileum. The segments were processed, embedded, and stained to make tissue sections, the villus height （VH） and the associated crypt depth （CD）, villus width （WD） were measured, and then the ratio of villus height to crypt depth （VH/CD） and villous surface area （VSA） were calculated. The results showed that, in the experiment 1 and 2,with the increasing of the AKG supplement levels, D-xylose content was increased （P〈0.05）. D-xylose content was the highest （quadratic, P〈0.05） when the levle of AKG was 1.0%. In the experiment 1, with the increasing of AKG supplement levels, the activity of DAO in plasma was decreased （P〈0. 01）. The activity of DAO was the lowest （quadratic, P〈0. 05） with AKG level of 1.0%. In the experiment 3, compared with the control group, the VH/CD of the villus in all segments of small intestine of the group with supplement of 1.0% AKG decreased significantly （P〈0.05）. These results indicated that the supplement of AKG in the diet could improve histological morphology and function of the small intestine of

关 键 词：仔猪 Α-酮戊二酸 D-木糖 二胺氧化酶 内毒素 小肠黏膜形态 

分 类 号：S828.5[农业科学—畜牧学] O636.1[农业科学—畜牧兽医]