LDH释放法检测肿瘤患者PBMC和CIK细胞的细胞毒活性  

Measurement of Cytotoxic Activity of PBMC and CIK from Cancer-bearing Patients Using LDH Release Assay

作　　者：张蕾[1] 李芳秋[1] 张士新[1] 黄海嵘[2] 李德闵[2] 

机构地区：[1]南京军区南京总医院解放军临床检验医学研究所,南京210002 [2]南京军区南京总医院心胸外科,南京210002

出　　处：《现代检验医学杂志》2008年第6期27-29,共3页Journal of Modern Laboratory Medicine

摘　　要：目的建立检测细胞毒活性的乳酸脱氢酶(LDH)测定法,了解肿瘤患者外周血单个核细胞(PBMC)和细胞因子诱导的杀伤细胞(CIK)的抗肿瘤活性,为肿瘤的免疫细胞治疗提供参考。方法采集肿瘤患者和健康成人外周血,常规分离PBMC,体外进行CIK的培养,用LDH释放法分别检测PBMC和CIK对肿瘤细胞K562的细胞毒活性。结果肿瘤患者PBMC对K562的杀伤活力比正常人显著降低(P<0.05)。经多种细胞因子诱导培养7～10d后,健康人和肿瘤患者的CIK对K562的杀伤活力都有显著提高(P<0.01),肿瘤患者CIK杀伤活力接近于健康人。结论肿瘤患者PBMC细胞毒活性显著降低,但经诱导培养成CIK后则显著提高。用LDH释放法检测肿瘤病人CIK细胞毒活力,有助于CIK疗法适应病人的选择及个体疗效观察。Objective To investigate the anti-tumor activity of peripheral blood monouclear cells （PBMC） and CIK cells from cancer-bearing patients. Methods PBMC from healthy donors and patients were isolated and were incubated with various types of cytokines to generate CIK. Cytotoxic activity of PBMC and CIK from cancer-bearing patients at diagnosis was evaluated using an LDH release assay. Results The anti-tumor activity of PBMC from patients was much lower than that of healthy donors. After stimulation, the cytotoxic activity of CIK was significant increased,and the cytotoxic activity in CIK was increased to the proximate level of these two groups,respectively. Conclusion Results show the enhancement of cytotoxic activity of CIK cells from both healthy donors and cancer patients. LDH release assay is useful for choosing candidated patients and for estimating the individual therapeutic efficiency of CIK.

关 键 词：乳酸脱氢酶释放法 细胞因子诱导的杀伤细胞 细胞毒活性 肿瘤治疗 

分 类 号：R730.43[医药卫生—肿瘤]