致倦库蚊Cecropin基因克隆及序列分析  

Cloning and Sequence Analysis of A Cecropin Gene of Culex quinquefasciatus

作　　者：康哲[1] 张春林[1] 陈汉彬[1] 

机构地区：[1]贵阳医学院生物学教研室,贵州贵阳550004

出　　处：《贵阳医学院学报》2008年第6期643-646,651,共5页Journal of Guiyang Medical College

基　　金：贵州省科学技术基金资助项目[黔科合丁字(2006)2077号]

摘　　要：目的:克隆致倦库蚊Cecropins基因序列,并进行生物信息学分析。方法:选取致倦库蚊成虫,提取其DNA并以此为模板,设计一对引物,采用PCR技术扩增出Cecropins基因,应用相关生物信息学软件分析其序列。结果:克隆出致倦库蚊Cecropins基因,并测定其扩增产物序列,经同源对比确认,进行了相关序列分析。结论:获得致倦库蚊Cecropins基因序列,基因长度为259bp,与尖音库蚊一致性为97%。Objective： To clone and analyze the sequence of cecropin gene of Culex quinquefasciatus. Methods ： Genomic DNA of C. quinquefasciatus adults was isolated, and was used as template to clone cecropin gene by PCR with a pair of designed primers ATGAACTrCAACAAGCTTF and CAAGTC-CTACT TYCCAAGT. The obtained sequence was analyzed with bioinformatics softwares. Results： A cecropin gene of C. quinquefasciatus was obtained, and was sequenced and analyzed. The results showed that the gene was 259 bp and was 97% consistent with that of cecropin gene of C. pipiens. Conclusion： The cloning is successful, and one of the cecropin genes of C. quinquefasciatus was obtained.

关 键 词：库蚊属 基因cecropin 基因顺序 

分 类 号：R696.442[医药卫生—泌尿科学]