关中马mtDNA D-loop区遗传多态性分析  

Analyses on mtDNA D-loop Genetic Diversity in Guanzhong Horse

作　　者：苏锐[1] 谢文美[1] 张云生[1] 王小斌[1] 张春梅[1] 王美菊[1] 陈宏[1,2] 雷初朝[1] 

机构地区：[1]西北农林科技大学动物科技学院陕西省农业分子生物学重点实验室,陕西杨凌712100 [2]徐州师范大学细胞与分子生物学研究所,江苏徐州221116

出　　处：《西北农业学报》2008年第6期17-20,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：教育部新世纪优秀人才支持计划(NCET-07-0699);西北农林科技大学科研专项(07ZR004)

摘　　要：利用测序技术和生物信息学分析技术,对关中马27个个体的线粒体DNA D-loop 247 bp序列的遗传多态性及系统进化进行分析,检测到9种单倍型,其核苷酸多态位点19个,约占所测核苷酸总长的7.69%,均为转换。关中马mtDNA D-loop区核苷酸多样度(π值)为0.0219±0.0076,单倍型多样度(H)为0.843±0.041,表明关中马mtDNA遗传多态性丰富。根据mtDNA序列构建了关中马的NJ分子系统树,发现存在A、C、D、F和G共5个支系,表明关中马是多起源的。The genetic diversity and phylogenetic evolution of 247 bp mitochondrial DNA （mtDNA） D- loop sequences of 27 Guanzhong horse samples were analyzed by sequencing and bioinformation techniques. The results revealed 9 mitochondrial haplotypes with 19 polymorphic sites, covering 7.69% of the analyzed length of the 247 bp D-loop sequences. Among the polymorphic sites, there were all transitions. The nucleotide diversity （π value） and haplotype diversity （H） estimated were 0. 0219±0. 0076 and 0. 843±0. 041, respectively, showing high mitochondrial genetic diversity in Guanzhong horse breed. The constructed Neighbor - Joining molecular phylogenetic tree based on the 9 mtDNA D loop haplotypes, indicated that lineages A, C, D, F and G were detected which showed multiple maternal origins in Guanzhong horse breed.

关 键 词：关中马 MTDNA D-LOOP 多态性 单倍型 支系 

分 类 号：S821[农业科学—畜牧学]