光敏生物素标记IgG检测农产品重金属污染  

Measuring Metal Ions Pollution of Farm Product by Photobiotin-labelled IgG

作　　者：邹亚丽[1] 王廷璞[1] 张娟[1] 

机构地区：[1]天水师范学院生命科学与化学学院,甘肃741001

出　　处：《西北农业学报》2008年第6期289-293,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：天水师范学院科研基金资助项目(X4-18);甘肃省555人才基金资助项目(2005166)

摘　　要：重金属污染的植物体内会产生较高的金属硫蛋白(MT),实验以光敏生物素标记用MT免疫家兔产生的抗体,用碱性磷酸酶标记亲和素,对MT进行免疫印迹检测,方法简单准确,标记的IgG最低检出量为1 ng/mL。通过对天水秦城区不同地域采集到的16种农产品样本进行检测,其检测结果与样本中金属硫蛋白的含量测定结果一致。Metallothionein can be induced in the plant by metal ions pollution. The Dot-bllotting assay is able to test metallothionein by alkaline phosphatase-labelled avidin , and photosensitive biotion-labelled antibody, which produced by domesticrabbit with the metallothionein immunity. The result showed that the lowest concentration of IgG is 1ng/mL that can be test. This method is simple and precision. 16 kinds of plants that are gathered in Qinzhou district of Tianshui city were measured by this method , the results was consistent with MT content of the samples.

关 键 词：重金属 金属硫蛋白 光敏生物素 抗体 

分 类 号：S63[农业科学—蔬菜学]