水样中氮磷营养盐的短期保存技术研究  

Investigations of the short-term preservation method of nitrogen and phosphorous in water samples

作　　者：徐善良[1] 沈勤[1,2] 严小军[1] 骆其君[1] 

机构地区：[1]宁波大学浙江省海洋生物工程重点实验室,浙江宁波315211 [2]桐乡水产工作站,浙江桐乡314500

出　　处：《海洋科学》2008年第12期52-59,共8页Marine Sciences

基　　金：欧盟第六框架计划项目资助(INCO-CT-2004-510706);教育部科技创新工程重大项目培育资金资助(705028)

摘　　要：用8种保存方法对淡水和海水水样中四个氮磷营养盐(NH3-N、NO2-N、NO3-N、PO4-P)参数及总氮的稳定性进行了7d保存效果的比较研究。结果表明,淡水与海水氮磷营养盐的最佳保存方法与效果各不相同,但淡水和海水的总氮值在各种方法保存下均十分恒定。综合考虑有效性及可操作性后提出了5d内的短期保存技术:(1)海水水样用5‰氯仿4℃条件下保存,供NH3-N、NO2-N和NO3-N三个营养盐的测定,用5‰甲醛4℃条件下保存供PO4-P测定;(2)淡水用5‰氯仿4℃条件下保存,供NH3-N测定,5‰甲醛4℃条件保存供NO2-N、NO3-N和PO4-P测定。Parameters of nitrogen, phosphorous（NH3-N, NO2-N, NO3-N, PO4-P） and stability of total nitrogen in freshwater and seawater samples in 7 days were comparitively studied to establish a suitable method from eight preservation ones. As the result, the optimal method and effect of preservation were different. But in both seawater and freshwater, the stability of total nitrogen turned out to be constant. Considering effectiveness and maneuverability, the short term preservation methods in 5 days were put forward for.. （1） the seawater samples were preserved by 5‰ chloroform at 4℃ for determining of three nutrients （NH3-N, NO2-N,and NO3-N）, and was preserved by 5‰ formaldehyde at 4℃ for determining of PO4-P; （2） the freshwater samples were preserved by 5‰ chloroform at 4℃ for determining of NH3-N, and were preserved by 5%0 formaldehyde at 4℃ for determining of three nutrients （NO2-N,NO3-N, PO4-P）.

关 键 词：氮 磷 营养盐 保存 氨氮 亚硝态氮 

分 类 号：X832[环境科学与工程—环境工程]