食管扩张刺激后兔食管内脏感觉改变及其中枢机制的实验研究  

Experimental study on changes of esophageal visceral sense stimulated by esophageal distention in rabbits and its mechanisms

作　　者：许树长[1] 陈莹[1] 王琛[1] 来立人[1] 李雷佳[1] 孔弦[1] 王志勇[1] 陈锡美[1] 

机构地区：[1]上海同济大学附属同济医院消化科,200065

出　　处：《中华消化杂志》2008年第11期759-763,共5页Chinese Journal of Digestion

摘　　要：目的探讨食管扩张刺激后兔食管内脏感觉改变及P物质（SP）、降钙素基因相关肽（CGRP）、5-羟色胺（5-HT）、Fos蛋白在中枢神经系统的作用。方法新西兰白兔20只分为三组：食管扩张组（A组，n=8）和对照组（B组，n=6），分别给予0．9cm食管球囊扩张及假手术刺激，每次持续30S，每日2次，共14d，以及空白对照组（C组，n=6）。采用动物行为学评分评价食管内脏感觉改变，免疫组化法观察幼兔食管下段黏膜、脊髓和脑组织中神经递质CGRP、SP、5HT及Fos蛋白的表达。结果动物食管机械扩张后，在行为学评分为1、2、3分时，A组球囊直径较B、c组明显减小（P〈0．05）；A组食管、脊髓和延髓孤束核（NTS）的SP表达明显高于B、C组（P％0．05），而B、C组间差异无统计学意义（P〉0．05）；A组食管黏膜、脊髓、NTS、中脑导水管周围灰质（PAG）、丘脑的CGRP、Fos阳性细胞数较B、C组明显增多（P〈0．05）；在食管和脊髓，A组5-HT的表达较B、C组显著升高（食管：27．67±3．27比11．00±1．79和11．17±1．33；脊髓24．00±5．22比11．33±2．94和11．83±2．48，P〈0．01），而在PAG，B组（17．67±2．07）和C组（16．83±2．32）5HT的表达较A组（13．17±2．04）增多（P％0．05）。在脊髓，CGRP与Fos、SP与Fos、cGRP与SP有明显的相关性（相关系数分别为0．813、0．779、0．772，P值分别为0．025、0．034、0．036）。结论持续的食管扩张可引起食管内脏敏感性增高，食管的机械扩张刺激通过脊髓、NTS、PAG、丘脑传导，且SP、CGRP、5-HT等在食管内脏感觉发生中发挥着重要作用。Objective To investigate the changes of esophageal visceral sence stimulated by esophageal distention in rabbits and the protein expressions of calcitonin gene related peptide （CGRP）, P substance （SP）, 5-hydroxytryptamine （5-HT）, Fos protein in central nervous system （CNS）. Methods Twenty New Zealand rabbits were randomly divided into experimental group （n = 8, received esophageal distention with 0.9 cm balloon for 30 s twice a day for 14 days ） ,control group （n = 6 ,received esophageal stimulation without balloon for 30 s twice a day for 14 days ）and blank control grouop （n =6）. The esophageal visceral sense was evaluated by animal behavior scores. The expressions of SP, CGRP, 5-HT and Fos protein esophagus mucosa, spine, nucleus tractus solitari （NTS）, periaqueductal gray （PAG） and thalamus were measured by immunochistochemistry. Results At the same behavior scores, the tube diameter of experimental group was significant lower than those of control group （P〈0.05）. The expression of SP in esophagus mucosa, spine and NTS was significant increased in experimental group compared to two control groups（P〈0.05）. The expression of CGRP and Fos in esophagus mucosa, spine, NTS, PAG and thalamus was increased in experimental group compared to two control groups （P〈0. 05）. The expression of 5-HT in esophagus mucosa and spine was higher in experimental group than that in control and blank control groups （esophagus： 27.67 ± 3.27 vs 11.00±1.79 or 11.17±1. 33;spine：24.00±5.22 vs 11.33±2.94 or 11.83±2.48,P〈 0.01）. But the expression of 5-HT in PAG was lower in experimental group（13.17±2.04） than that in control 17.67±2.07）and blank control （16.83±2.32）groups （P〈0.05）. There was significant correlation between CGRP and Fos, SP and Fos, CGRP and SP in spine （r=0. 813,0. 779,0. 772,P 0. 025,0. 034, 0. 036, respectively）. Conclusions Esophageal hypersensitivity may be induced by esophageal distention. The expresstion of SP, CGRP, 5-HT was

关 键 词：胃食管反流病 食管内脏高敏感 神经递质 动物实验 

分 类 号：R686[医药卫生—骨科学]