超氧化物歧化酶和PLUNC蛋白在慢性鼻窦炎和鼻息肉组织中的表达及意义  

Expression and significance of Cu/ZnSOD and PLUNC protein in chronic sinusitis and nasal polyp tissue

作　　者：吴敏曼[1] 孙虹[2] 刘火旺[2] 王庭阔[2] 李昌琪[3] 刘丹[3] 

机构地区：[1]云南中医学院第一附属医院耳鼻咽喉科,昆明650021 [2]中南大学湘雅三医院耳鼻咽喉头颈外科 [3]中南大学湘雅医学院人体解剖学与神经生物学系

出　　处：《临床耳鼻咽喉头颈外科杂志》2008年第20期913-916,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：教育部博士点基金资助项目(No:20030533006);湖南省自然科学基金资助项目(No:01JJY2092);2004年中南大学博士研究生学位论文创新选题立项资助项目(No:040145);湘雅三医院2005年博士化基金资助项目(No:20050328)

摘　　要：目的:采用免疫组织化学实验并扩大标本量来进一步验证超氧化物歧化酶(Cu/ZnSOD)和PLUNC蛋白在鼻息肉、慢性鼻窦炎和正常鼻黏膜组织之间的差异表达及意义。方法:采用免疫组织化学SP法分别检测34例鼻息肉、30例慢性鼻窦炎和18例正常鼻黏膜组织中Cu/ZnSOD和PLUNC蛋白的表达情况。结果:①Cu/ZnSOD和PLUNC蛋白在同一个组织标本中的定位和表达强度一致,均表达于鼻黏膜上皮、腺上皮和杯状细胞中;②鼻息肉、慢性鼻窦炎和正常鼻黏膜组织之间Cu/ZnSOD和PLUNC蛋白阳性强度构成比的差异有统计学意义(χ2=30.689,P<0.01)。鼻息肉组织的阳性强度以"+"为主,慢性鼻窦炎组织的阳性强度以"+++"为主,正常鼻黏膜组织的阳性强度以"++"为主。结论:Cu/ZnSOD和PLUNC蛋白是鼻黏膜局部免疫防御系统的重要组成部分。当鼻黏膜受病原体侵袭或理化因素刺激引起炎症反应时,鼻黏膜上皮、腺上皮和杯状细胞反应性增生明显,它们产生和分泌Cu/ZnSOD、PLUNC蛋白等抗炎物质增多来产生炎症防御作用。Objective.. To validate the expression of Cu/ZnSOD and PLUNC protein in chronic sinusitis and nasal polyp tissue by immunohistochemistry. Metbod：Thirty four nasal polyps, 30 chronic sinusitis and 18 control cases were chosen. All samples were stained with SP immunohistochemical method to examine Cu/ZnSOD and PLUNC protein respectively. Result： （1）Interestingly, we found the positive intensity of Cu/ZnSOD and PLUNC protein were the same in one sample, and they were both expressed in nasal epithelium mucosae, glandular epithelium and goblet cells; （2）We calculated that the constituent ratio among nasal polyps, chronic sinusitis and normal nasal mucosa was significant difference （2 = 30. 689, P〈0.01）. The positive intensity of nasal polyps was mainly ＂＋＂, and the positive intensity of chronic sinusitis was mainly ＂＋＋＋＂, and the positive intensity of normal na- sal mucosa was mainly ＂＋＋＂. Conclusion： Both Cu/ZnSOD and PLUNC protein are important part of the innate defense mechanism in nasal mucosa. Once the nasal mueosa is irritated by infectious agents or chemical factors, na- sal epithelium mueosae, glandular epithelium and goblet cells will proliferate reactively, and then produce antiinflammatory agents such as Cu/ZnSOD and PLUNC protein to defend inflammation.

关 键 词：鼻窦炎 鼻息肉 超氧化物歧化酶 PLUNC蛋白 

分 类 号：R765.4[医药卫生—耳鼻咽喉科]