中国HIV-1 C／B’重组毒株和B’毒株感染者Nef特异性T细胞免疫应答的研究  

Nef-specific T lymphocyte responses in Chinese HIV-1 recombinant subtype C/B＇ and subtype B＇ infectors

作　　者：刘宏伟 洪坤学[2] 马军[3] 袁霖[2] 刘沙[2] 陈健平[2] 张远志[3] 阮玉华[2] 王哲 邵一鸣 

机构地区：[1]河南疚病预防控制中心性病艾滋病研究所,郑州450016 [2]中国疾病预防控制中心艾滋病中心病毒免疫室,北京100050 [3]新疆疾病预防控制中心

出　　处：《中华微生物学和免疫学杂志》2008年第11期998-1003,共6页Chinese Journal of Microbiology and Immunology

摘　　要：目的研究中国主要流行的HIV-1 C／B’重组毒株和B’亚型毒株感染者Nef特异性T细胞反应特征，确定两种亚型感染者共同识别的免疫优势区。方法本研究以59名HIV-1 C／B’重组毒株、27名B’亚型毒株感染者为研究对象，用ELISPOT检测针对HIV-1型C／B’Nef重叠多肽产生IFN-γ的特异性T细胞反应。结果44例（74．58％）HIV-1 C／B’重组毒株感染者产生Nef特异性T细胞反应，主要识别EVA7081．1、5、6、7、43、44、45、47、48、49这10条多肽，氨基酸序列为Nef63～115和117～139的区域。20例（74．07％）的HIV-1 B’毒株感染者产生Nef特异性T细胞反应，主要识别EVA7081．1、2、43、49这4条多肽，氨基酸序列为Nef63～77和87～119的区域。两种亚型感染者特异性T细胞反应的强度和广度与病毒载量和CD4细胞数不相关。结论中国HIV-1 C／B’重组毒株和B’亚型毒株感染者共同识别氨基酸序列为Nef63—77和87～115的免疫优势区，提示此区域可用于疫苗的设计．Objective To analyze character of Nef-specific T lymphocyte responses in Chinese HIV-1 recombinant subtype C/B＇ and subtype B＇ infectors and to identify the common immunodominant regions recognized by these infectors. Methods Fifty-nine HIV-1 recombinant subtype C/B＇ infectors and 27 subtype B＇ infectors were tested by IFN-γ enzyme linked immunospot （ELISPOT） assay using HIV-I C/B＇ Nef overlapping peptides. Results Nef-specific T-cell responses of IFN-γ secretion were identified in 44 （74.58%） out of 59 HIV-1 recombinant subtype C/B＇ infectors. Ten peptides, EVAT081.1, 5, 6, 7, 43, 44, 45, 47, 48, 49 were mainly recognized. Amino acid position was from Nef63 to 115 and 117 to 139. Twenty of 27 （74.07%） HIV-1 subtype B＇ infectors recognized peptides. EVA7081.1, 2, 43 and 49 were mainly recognized. Amino acid position was from Nef 63 to 77 and 87 to 119. There was no correlation between the Nef-speclfic IFN-production of HIV-1-specific T cells responses and viral load or CD4 T cell count in both subtype infectors. Conclusion The immunodominant regions, from Nef 63 to 77 and 87 to 115 were recognized by both Chinese HIV-1 recombinant subtype C/B＇ infectors and subtype B＇ infectors. These regions could be used in design of vaccine.

关 键 词：人免疫缺陷病毒 NEF 特异性T细胞反应 酶联免疫斑点试验(ELISPOT) γ-干扰素 

分 类 号：R512.91[医药卫生—内科学] R392[医药卫生—临床医学]