云南高致病性猪繁殖与呼吸综合征病毒Nsp2基因变异分析  

Gentic variation of Nsp2 genes of highly pathogenic porcine reproductive and respiratory syndrome viruses in Yunnan province

作　　者：庞海洋[1] 赵焕云[2] 张文东[3] 张思东[3] 吕粤[3] 岳亮[3] 范泉水[4] 张应国[3] 张富强[4] 

机构地区：[1]云南农业大学,云南昆明650203 [2]西北农林科技大学,陕西杨凌712100 [3]云南省动物疫病预防控制中心,云南昆明650051 [4]成都军区疾病预防控制中心,云南昆明650032

出　　处：《中国预防兽医学报》2008年第12期934-938,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：云南省后备人才基金项目(2005PY01-12)

摘　　要：采用RT-PCR技术对云南2007年采集的48份猪组织样品中病毒Nsp2基因进行检测,获得阳性样品14份,选择5份代表性样品将其PCR产物纯化后,克隆至pMD18-T载体测序,并与已知代表性毒株进行比较及系统发育分析。结果发现云南病毒样品Nsp2基因核苷酸及氨基酸序列同源性分别为95.8%～99.0%和92.3%～98.6%。5份样品病毒Nsp2蛋白482位、534位～562位氨基酸均存在缺失,其中1份样品(YNMG)486位～489位氨基酸也发生了缺失。云南病毒样品在系统发育树上可划分为4个亚组群,分别与广东、广西、贵州、浙江、江苏、山东毒株遗传关系密切。The highly pathogenic porcine reproductive and respiratory syndrome, characterized by high fever and high morbidity and mortality in pigs, is a severe infectious disease caused by variant strains of porcine reproductive and respiratory syndrome virus （PRRSV）. The Viral Nsp2 protein is a potential virulence-associated factor. In this study, 48 swine tissue samples collected from Yunnan province during 2007 were screened by RT-PCR for Nsp2 gene and 14 samples detected positive. The PCR products from 5 isolates were sequenced and compared with those of representative PRRSV strains. The results showed that the Nsp2 genes detected in Yunan shared 95.8 % to 99.0 % identity at nucleotide acid level and 92.3 % to 98.6 % at amino acid level. There were deletions at position aa482 and aa534 to aa562 on Nsp2 protein in all Yunnan isolates. A continuous deletion of 4 residues from aa486 to aa489 in Nsp2 protein was also found in one Yunnan isolates （YNMG）. Phylogenetic analysis showed that those Yunnan samples could be divided into 4 different subgroups and genetically related to virus isolates from Guangdong, Guangxi, Guizhou, Zhejiang, Jiangsu and Shandong provinces.

关 键 词：猪繁殖与呼吸综合征病毒 高致病性 NSP2基因 变异 

分 类 号：S855.3[农业科学—临床兽医学]