重型乙型肝炎血清白细胞介素-15水平检测及临床意义  

Clinical significance of serum interleukin-15 in the patients of severe type B hepatitis

作　　者：陈定贵[1] 张一[2] 张锁才[1] 罗德宝[1] 

机构地区：[1]江苏省常州市第三人民医院肝病科,213001 [2]南昌大学第一附属医院感染科

出　　处：《中国综合临床》2008年第12期1215-1217,共3页Clinical Medicine of China

基　　金：江西省科学技术厅社会发展攻关项目（2004）

摘　　要：目的观察重型乙型肝炎患者血清白细胞介素-15（IL-15）水平的变化，探讨其在重型肝炎发病机制中的可能作用及临床意义。方法采用双抗体夹心酶联免疫吸附试验检测47例重型乙型肝炎和20例健康者血清IL-15水平，同期检测血清丙氨酸氨基转移酶（ALT）、总胆红素（TBIL）、凝血酶原活动度（PTA）等，分析重型乙型肝炎患者血清IL-15水平与ALT、TBIL、PTA等的相关性。结果重型乙型肝炎组血清IL-15为（18．94±7．5）ng／L，显著高于正常对照组（5．9±2．0）．g／L（P〈0．01）；死亡组血清IL-15为（24．14±7．5）ng／L，显著高于存活组（15.7±5．4）ng／L（P〈0．01）；存活组患者血清IL-15浓度随一般情况好转、肝功能恢复而下降。此外，重型乙型肝炎组IL-15与TBIL呈正相关（r=0．570，P〈0．01），与PTA呈负相关（r=-0．529，P〈0．01），与ALT则无显著相关性（r=0．099，P〉0．05）。结论IL-15可能参与重型乙型肝炎的发病过程。IL-15水平与病情存在一定程度的一致性，与病情的转归密切相关。血清IL-15水平的检测可能对于重型乙型肝炎患者的预后具有预测作用。Objective To observe the level changes of interleukin-15 （IL-15） and its role and clinical significance in severe type B hepatitis （ HB ）. Methods IL-15 levels of 47 cases of severe HB and 20 eases of healthy subjects were detected by ELISA, meanwhile the alanine aminotransferase （ ALT ）, total bilirubin （TBIL ）and prothrombase activity （ PTA） were measured as well. The correlation between IL-15 and ALT,TBIL and PTA were analyzed. Results IL-15 in severe HB cases were higher than in control group [ （ 18.9 ± 7.5 ） ng./L vs. （ 5.9 ± 2.0 ） ng/L,P 〈0.01 ] ,which was higher in death group than in survival group[ （ 24.1 ± 7.5 ） ng/L vs. （ 15.7 ± 5.4 ） ng/L,P 〈0.01 ]. IL-15 level was decreasing with the improvement of general condition and liver function recovery. In addition, IL-15 in severe HB was positively correlated with TBIL （ r = 0. 570,P 〈 0.01 ） and negatively correlated with PTA（ r = -0.529 ,P 〈0.01 ） but was not significantly correlated with ALT（ r =0.099 ,P 〉0.05 ）. Conclusion IL-15 may take part in the pathogenesis of severe HB,which is consistent with disease condition and is closely re- lated to the improvement of disease. The detection of IL-15 may exert a predicting role in the prognosis of severe HB.

关 键 词：乙型肝炎 白细胞介素-15 酶联免疫吸附试验 

分 类 号：R512.62[医药卫生—内科学]