光敏开关及其在化学生物学中的应用  

Photolysis of Caged Compounds and Its Applications to Chemical Biology

作　　者：孟祥明[1,2] 陈孝云[1] 傅尧[1] 郭庆祥[1] 

机构地区：[1]中国科学技术大学化学系,合肥230026 [2]安徽大学化学系,合肥230039

出　　处：《化学进展》2008年第12期2034-2044,共11页Progress in Chemistry

基　　金：国家自然科学基金项目(No.90713009);安徽省高校自然科学研究计划项目(No.KJ2008B159)资助

摘　　要：近年来,随着生物医学技术的发展,人们需要越来越细致地在分子水平上研究各种生命过程。为了能够实现实时原位地观察活细胞或组织中的生命化学过程,需要使用以物理方法来选择性激活的分子探针。以共聚焦激光技术为基础的光敏开关能很好地解决这一问题。迄今,发展和用于光敏开关的光敏剂已成为化学生物学研究的重要方向。本文重点总结了各种可应用于共聚焦激光系统的单、双光子光敏剂及其光解机制(单光子光敏剂主要有:硝基苯类、香豆素类等;双光子光敏剂主要有:香豆素类、喹啉类及吲哚衍生物类)以及这几类光敏剂在化学生物学中的应用。The recent development in biomedical technology demands more accurate understanding of biological processes at molecular level. In order to observe the chemical processes of living cells or tissues in real-time fashion with high space resolution, it is necessary to develop methods to selectively activate molecular probes in living cells. Confocal laser technology provides a very efficient method for activation of the molecular probes without any injury to tissues. Based on this technology, a series of photon switch which have biological applications have been realized. By now, the development of caging groups for photon switch has become an interesting research area. In this paper, we summarize the most popular photo-removable caging groups including nitrobenzene, eoumarin, indole derivatives, etc. The mechanism of these caging groups using as photon switch is surveyed. The application and the prospects of these groups in chemical biology are also discussed.

关 键 词：光子开关 共聚焦激光 光敏剂 

分 类 号：O657.3[理学—分析化学]