毛细管电泳-电致化学发光法测定氢溴酸右美沙芬  

Determination of dextromethorphan hydrobromide in oral solution by capillary electrophoresis with electrochemiluminescence detection

作　　者：彭志兵[1] 吴正平[1] 

机构地区：[1]宜春学院医学美学研究所,江西宜春336000

出　　处：《化学试剂》2008年第12期915-918,共4页Chemical Reagents

摘　　要：基于氢溴酸右美沙芬增强联吡啶钌的电致化学发光信号，研究了采用毛细管电泳-电致化学发光法分离检测氢溴酸右美沙芬含量的新方法。利用未涂层石英毛细管44cm×25μm；分离缓冲溶液为10mmo／L磷酸盐缓冲溶液（pH7．5）；分离电压15kV；检测池中溶液为50mmo／L磷酸盐缓冲溶液（pH7．5）和5mmo／L联吡啶钌。在2008内可实现氢溴酸右美沙芬的分离检测，其线性范围为1．0×10^-7～1．0×10^-5mol／L，相关系数为0．9993，检出下限为1．9×10^-8mol／L。本法操作简便快速、灵敏度高、结果准确可靠，可用于氢溴酸右美沙芬口服液中氢溴酸右美沙芬的质量监测。A novel and sensitive method for determination of dextromethorphan hydrobromide in oral solution has been studied by using capillary electrophoresis （CE） coupled with electrochemiluminescence （ECL） detection,based on the ECL enhancement of tris（2,2＇-bipyridyl） ruthenium （ Ⅱ ） with the dextromethorphan analyte. The effects of several factors such as the concentration of tris（2,2＇-bipyridyl） ruthenium （ Ⅱ ）, the detection potential, the concentration and the pH of phosphate buffer, the electrokinetic voltage and the injection time were investigated. Under the optimum conditions, dextromethorphan hydrobromide in oral solution could be separated and detected within 200 s. The linear concentration of dextromethorphan hydrobromide ranged from 1.0 × 10^-7 to 1.0 × 10^-5 mol/L （with a correlation coeflqcient of 0.999 3） .The limit of detection was 1.9 × 10^-8 mol/L （S/N = 3）. This method is simple and quick in operation, sensitive and reliable in analytical results and it can be used for the quality control over dextromethorphan hydrobromide in oral solution.

关 键 词：氢溴酸右美沙芬 毛细管电泳 电致化学发光 联吡啶钌 

分 类 号：O657.8[理学—分析化学]