半夏提取液对大豆蚜虫生物活性及活性剂量的初步筛选  

Biological Activity of Pinellia ternata with Various Extraction against Aphis glycines and Preliminary Screening of Activity Concentration

作　　者：顾地周[1] 车喜全[2] 王立斌[2] 何晓燕[1] 朱俊义[1] 赵玉敏[1] 

机构地区：[1]通化师范学院生物系,吉林通化134002 [2]通化师范学院化学系,吉林通化134002

出　　处：《农药》2008年第12期927-929,共3页Agrochemicals

基　　金：吉林省教育厅自然科学基金资助项目[(2003)-65]

摘　　要：以半夏的球根茎为试材,基于均匀设计法研究了半夏球根茎的不同提取液对大豆蚜虫的触杀和拒食作用。结果表明:乙醇浸提液对大豆蚜虫具有较高的触杀作用。水蒸气蒸馏液对大豆蚜虫具有较强的拒食作用。乙醇浸提液对大豆蚜虫48h的致死中浓度(LC50)为9.7439g/L,水蒸气蒸馏液对大豆蚜虫24、48h的拒食中浓度(AFC50)分别为8.3756、7.9162g/L。丙酮浸提液对大豆蚜虫的触杀和拒食作用不显著。半夏球根茎的乙醇浸提液和水蒸气蒸馏液对大豆蚜虫54h的同时触杀和拒食活性质量浓度初步筛选结果表明:乙醇浸提液24.1955g/L和水蒸气蒸馏液23.0230g/L联合使用对大豆蚜虫同时触杀和拒食活性作用最好,死亡率达100%。The bulbs of Pinellia ternata were used as the experimental material, uniform design for studied various extraction for contacting and antifeeding against Aphis glycines. The results showed that ethanol extract possessed high effectiveness of contacting against Aphis glycines, the LC50 of death was 9.7439 g/L in 24 h. Wetdistillation liquid possessed strong effect on antifeeding of Aphis glycines, the AFC50 of antifeeding were 8.3756, 7.9162 g/L in 24, 48 h respectively. Acetone extracts had indistinctively effect on contacting and antifeeding of Aphis glycines. The results of preliminary screening for contacting and antifeeding against Aphis glycines showed that ethanol extract with 24.1955 g/L and wetdistillation liquid with 23.0230 g/L had strong effect on contacting and antifeeding of Aphis glycines simultaneously, the rate of death was 100% in 54 h.

关 键 词：半夏 大豆蚜虫 均匀设计 杀虫活性 

分 类 号：S482.3[农业科学—农药学]