β3GalT7基因转染HL-60细胞及对其生物学行为的影响  

Transient transfection of HL-60 with β3GalT7 gene and its influences on the biological behaviors of transfected cells

作　　者：行书丽[1] 岳爱环[1] 唐春花[1] 贾伟[1] 吴士良[1] 

机构地区：[1]苏州大学基础医学与生物科学学院生物化学与分子生物学教研室苏州大学医学部生化工程研究所,江苏苏州215123

出　　处：《江苏大学学报（医学版）》2008年第6期503-506,I0001,共5页Journal of Jiangsu University：Medicine Edition

基　　金：国家自然科学基金资助项目(30670462)

摘　　要：目的:初步探讨人类β1,3半乳糖基转移酶7(β3GalT7)基因对HL60细胞的细胞周期、侵袭能力等方面的影响。方法:通过脂质体介导将本实验室构建好的β3GalT7真核正义表达载体pEGFP-C1-T7-Sense和反义表达载体pEGFP-C1-T7-AntiSense转染入HL60细胞;流式细胞仪分析各细胞株的细胞周期改变;Transwell侵袭实验检测β3GalT7对HL60细胞侵袭转移能力的影响。结果:随着β3GalT7表达的增高,G2+S期细胞增多,且细胞侵袭能力增强;而转染反义表达载体pEGFP-C1-T7-AntiSense的结果是相反的。结论:过度表达β3GalT7的HL-60细胞株体外增殖和侵袭能力增强。Objective： To explore the effects of β3 GalT7 gene on cell cycle, invasive potential of HL- 60. Methods： Transfect the recombinant plasmids pEGFP-Cl-T7-Sense and pEGFP-Cl-TT-AntiSense into HL-60 with liposomes. Cell cycle and invasive potential of the transfected cells with flow cytometry and transwell plate, respectively. Results： As the up-regulation expression of β3GalTT, the cell growth rate increased. At 48 h after transfection, significantly increased invasiveness was noted in HL60 transfected with pEGFP-Cl-T7-Sense. Conclusion： Over-expressing/β3Gal77 in HL-60 enhanced proliferative and invasive potential.

关 键 词：β1 3半乳糖基转移酶7 正义表达载体 反义表达载体 细胞周期 侵袭 

分 类 号：Q53[生物学—生物化学]