小麦胁迫相关基因W1的克隆及表达模式分析  

Cloning and Expression Analysis of a Stress-related Gene W1 in Wheat

作　　者：刘丽[1,2] 徐兆师[2] 张瑞越[1,3] 倪志勇[1,2] 陈耀锋[1] 李连城[2] 陈明[2] 马有志[2] 

机构地区：[1]西北农林科技大学农学院,杨凌712100 [2]中国农业科学院作物科学研究所／农作物基因资源与基因改良国家重大科学工程／农业部作物遗传育种重点开放实验室,北京100081 [3]江苏淮阴师范学院生物系,淮安223300

出　　处：《植物遗传资源学报》2008年第4期423-427,共5页Journal of Plant Genetic Resources

基　　金：国家高技术研究发展计划(“863”计划)项目(2007AA10Z130);国家自然科学基金项目(30700504)

摘　　要：应用噬菌体原位杂交技术从干旱胁迫诱导的小麦cDNA文库中克隆到一个胁迫诱导的基因片段W1。W1全长cDNA为901bp,其中,编码区长498bp,编码166个氨基酸。Southern杂交表明,W1是一个低拷贝基因。RT-PCR结果表明,W1受干旱、低温的诱导,但不受高盐的诱导。氨基酸序列分析发现W1有一个USP保守区(pfam00582)。同源性分析发现W1与一个水稻胁迫诱导蛋白(NM_001061239)的同源性为83%,但该类蛋白的功能尚无报道。W1是小麦第1个被克隆的胁迫相关蛋白基因,该基因的克隆有助于阐明小麦的抗逆机制,并为今后培育抗逆性小麦品种提供候选基因。A putative stress-induced gene, W1, was cloned from the cDNA library of drought-treated wheat seedlings by phage hybridization in situ. The full-length cDNA of W1 consists of 901bp and contains a 498bp open reading frame （ORF） encoding a 166 amino acid protein. Southern blot analysis indicated that W1 was a low-copy gene. RT-PCR analysis revealed that the expression of W1 was upregulated by drought and cold. Amino acid sequence analysis discovered that W1 had a conserved region of USP （pfam00582）. Phylogenetic analysis showed that W1 was 83% identical to a rice stress-induced protein （NM_001061239）. However, genes of the group involved in putative stress responses were not reported. W1 is the first isolated USP gene in wheat, which promote to clarify the stress-resistant mechanism and provide a candidate gene for improving wheat stress-tolerant cuhivars.

关 键 词：小麦 RT-PCR 克隆 诱导机制 非生物胁迫 

分 类 号：S512.1[农业科学—作物学]