用ELISA方法检测鸡蛋氟喹诺酮类药物残留的研究  

Determination of fluoroquinolones residues in eggs by ELISA method

作　　者：刘欢欢[1] 于学辉[1] 彭莉[2] 岳秀英[2] 杨松沛[2] 刘内生[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041 [2]四川省兽药监察所,四川成都610041

出　　处：《西南民族大学学报（自然科学版）》2008年第6期1182-1185,共4页Journal of Southwest Minzu University(Natural Science Edition)

摘　　要：针对鸡蛋中喹诺酮类药物的残留,采用间接竞争ELISA方法建立了检测手段.样品经提取净化后,以氟喹诺酮类药物多残留试剂盒为基础,建立了鸡蛋中喹诺酮类药物残留的检测方法.结果显示,在5～50μg/kg浓度范围内的平均添加回收率在70%以上,批内变异系数在10%以内,批间变异系数在15%以内,检测限为5μg/kg,低于国家规定的喹诺酮类药物在鸡蛋中的残留限,建立了一种快速的对鸡蛋中喹诺酮类药物残留的检测方法,能满足目前基层初筛工作的实际需要.An indirect competitive ELISA method is developed for the determination of fluoroquinolones（FQs） in eggs. After being purified and ：xtracted, the method is established on the base of indirect competitive ELISA kit. The results show that the average recovery rate is above 70% between the added concentration of 5-50μg/kg, the interassay variation coefficient is within 10%, the coefficient of variation in group is less than 15% and the detection limit is 5μg/kg, lower than the state standard of the limit of detection of fluoroquinolones in eggs, and establish a rapid detection method which is suitable for the routine qualitative analysis of the large scale samples to meet the practical need for veterinary drug residue test.

关 键 词：鸡蛋 氟喹诺酮类药物 残留检测 间接ELISA 

分 类 号：S859.84[农业科学—临床兽医学]