益气温阳护卫法对哮喘大鼠IL-4/STAT6信号通路的影响  

Effect of Yiqiwenyanghuwei method on signal pathway of IL-4/STAT6 in asthma rats

作　　者：余建玮[1] 薛汉荣[1] 洪广祥[1] 邱小华 邹叶青 程光宇[1] 付向春[1] 杨玉萍[1] 兰智慧[1] 

机构地区：[1]江西中医学院呼吸病研究所,南昌330006 [2]江西省分子医学研究中心,南昌330006

出　　处：《中华中医药杂志》2008年第12期1082-1084,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金资助项目(No.30560188);江西省自然科学基金(No.0540112)

摘　　要：目的:探讨益气温阳护卫法对哮喘大鼠白细胞介素4/信号传导子及转录激活子6(IL-4/STAT6)信号通路的调节作用。方法:50只SPF级雄性SD大鼠随机分为正常对照组、哮喘模型组、益气温阳护卫组、地塞米松组和卡介菌多糖核酸组,每组10只。采用TaqMan Realtime PCR测定哮喘大鼠Th细胞IL-4和STAT6的mRNA表达变化。结果:哮喘模型组IL-4和STAT6的mRNA表达显著高于正常对照组(P<0.01),益气温阳护卫组、地塞米松组和卡介菌多糖核酸组PBMC中IL-4和STAT6的mRNA表达显著低于哮喘模型组(P<0.01),且与正常对照组IL-4和STAT6的mRNA表达相当。结论:益气温阳护卫法能够抑制哮喘大鼠IL-4/STAT6信号通路基因的表达,该作用可能是调节Th1/Th2细胞亚群趋于平衡的重要机制。Objective： To explore the effect of Yiqiwenyanghuwei method on signal pathway of IL-4/STAT6 in asthma rats. Methods： Fifty SPF male SD rats were randomly divided into 5 groups： the normal control group, the asthma group, the Yiqiwenyanghuwei group, the Dexamethasone group, the BCG-PSN group .The mRNA expression of IL-4 and STAT6 in the PBMC of all groups were detected by TaqMan Real time PCR. Results： The mRNA expression of IL-4 and STAT6 in PBMC of asthma group was significantly higher than that of control group（P〈0.01）, the mRNA expression of IL-12 and STAT4 in PBMC of Yiqiwenyanghuwei group, the Dexamethasone group and the BCG-PSN group were significantly lower than that of asthma group（P〈0.01）, but corresponding with the normal control group. Conclusion： Yiqiwenyanghuwei method can inhibit gene expression of IL-4/STAT6 signaling pathway in asthma rats, the effect may be the important mechanism to regulate Th1 / Th2 cell subsets balance.

关 键 词：益气温阳护卫法 哮喘 IL-4 STAT6 

分 类 号：R259[医药卫生—中西医结合]