门静脉高压症脾功能亢进脾巨噬细胞Toll样受体2、4 mRNA表达变化的研究  

Expression of Toll-like receptor 2,4 mRNA of splenic macrophage in hypersplenism due to portal hypertension

作　　者：张澍[1] 李宗芳[1] 苏清华[1] 黄辰[2] 任松[1] 张伟[1] 蒋安[1] 杨安宁[1] 

机构地区：[1]西安交通大学医学院第二附属医院外科 [2]西安交通大学医学院环境与疾病相关基因教育部重点实验室

出　　处：《中华普通外科学文献（电子版）》2008年第6期19-21,共3页Chinese Archives of General Surgery(Electronic Edition)

基　　金：国家自然科学基金资助项目(30471672);教育部新世纪优秀人才支持计划(2004NCET-04-0932);教育部优秀青年教师资助计划项目(2003)

摘　　要：目的检测门静脉高压症(PH)脾亢脾和正常脾巨噬细胞(Mφ)中Toll样受体2、4(TLR2、4)mRNA的表达差异,为进一步深入探讨Toll样受体在PH脾亢发生中的作用奠定基础。方法选取门静脉高压症脾亢患者(均为慢性乙型肝炎患者)的手术切除脾脏(12例)为实验组,外伤性脾破裂患者的手术切除脾脏(4例)为正常对照组。贴壁培养法分离纯化脾脏组织Mφ,荧光定量PCR法对Mφ表面Toll样受体2、4mRNA的表达进行检测,并将两组结果进行统计学分析比较。结果与正常脾脏相比,PH脾亢脾MφTLR2、4mRNA的表达水平明显增强(TLR2:2.29±0.55vs1.06±0.53,P<0.05;TLR4:2.32±0.41vs1.01±0.14,P<0.01)。结论PH脾亢脾MφTLR2、4的mRNA表达水平明显升高,与蛋白水平免疫组化的结果一致,进一步支持了"内毒素血症→脾脏MφToll样受体活化→Mφ吞噬破坏血细胞增多"是PH脾亢发生可能机制的观点。Objective To investigate the different expression of Toll-like receptor 2, 4 （TLR2,4） mRNA on splenic macrophage （MФ,） in hypersplenism due to portal hypertension （PH）, providing the evidence for discussing the effects of TLR in pathogenesis of hypersplenism. Methods 12 patients with hypersplenism due to PH and 4 patients with traumatic rupture of spleen were regarded as hypersplenia and control group respectively. The expressions of TLR2, 4 were detected by real time-PCR. The results of two groups were compared. Results As compared to control group, the expression of TLR2,4 mRNA were increased significantly （TLR2：2.29 ±0.55 vs 1.06 ±0.53, P 〈0.05 ;TLR4：2.32±0.41 vs 1.01±0.14, P〈0.01）. Conclusion The expression of TLR2, 4mRNA increased significantly, consistent with the result of protein expression. It has been further supported that ＂endotoxemia→activation of TLRs→increase of phagocytosis of splenic MФ→hypersplenism＂, this may be one of the mechanism of hypersplenism due to PH.

关 键 词：门静脉高压症 脾功能亢进 巨噬细胞 TOLL样受体 聚合酶链反应 

分 类 号：R575.2[医药卫生—消化系统]