秦岭华山松3种小蠹虫的消化酶研究  

Digestion enzymes of three bark beetle species(Coleoptera:Scolytidae) in the Qingling Mountains

作　　者：王胜军[1] 陈辉[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2008年第12期142-148,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金重点项目(30730073);国家"十一五"科技支撑计划项目(2001BA509B1203)

摘　　要：【目的】华山松大小蠹、松六齿小蠹和油松四眼小蠹,是秦岭森林生态系统内危害华山松的重要小蠹种类,通过对上述3种小蠹虫虫体主要消化酶活性的分析,揭示其营养利用差异的原因。【方法】采用福林-酚试剂法、羧甲基纤维素钠盐法、碱溶液滴定法和碘-淀粉比色法,测定并比较华山松大小蠹、松六齿小蠹和油松四眼小蠹3种小蠹虫的蛋白酶、纤维素酶、脂肪酶和淀粉酶活性,同时探讨不同温度对华山松大小蠹成虫与幼虫虫体的蛋白酶、纤维素酶、淀粉酶和脂肪酶活性的影响。【结果】华山松大小蠹、松六齿小蠹和油松四眼小蠹成虫虫体蛋白酶活性大小依次为:华山松大小蠹>油松四眼小蠹>松六齿小蠹;纤维素酶活性大小依次为:松六齿小蠹>油松四眼小蠹>华山松大小蠹;脂肪酶活性大小依次为:华山松大小蠹>油松四眼小蠹>松六齿小蠹;淀粉酶活性大小依次为:油松四眼小蠹>松六齿小蠹>华山松大小蠹。华山松大小蠹幼虫蛋白酶的最适温度为25℃,成虫为20℃;幼虫与成虫的纤维素酶、脂肪酶、淀粉酶的最适温度均一致,分别为25,25和40℃。【结论】3种小蠹虫的蛋白酶、纤维素酶、淀粉酶活性差异显著,脂肪酶活性差异不显著;华山松大小蠹成虫与幼虫的4种消化酶活性随温度的改变而变化。Abstract： [Objective] Dendroctonus arrnandi Tsai et Li, Polygraphus sinensis Eggers,and Ips acumi- natus Gyllenhal,are three main infested Chinese white pine bark beetles in Qinling Forest Ecosystem,and the differences of nutrition usage were discussed by analyzing its major digestive enzyme activities. [Method] Using forint phenol, carboxymethyl cellulose sodium salt,alkali solution titration methods and idodine starch color,digestive enzyme activities of the three were measured and compared and influences of different temperatures on the enzyme activities of D. armandi adults and larvae were investigated. [Result] The results indicated that the proteinase activity of D. armandi was the highest,followed by P. sinensis and I.acuminatus respectively. The cellulase activity of I. acu,ninatus ranked first,with P. sinensis and I. acuminatus in succession. The lipase activity comparison of the three was the same in asproteinase activity. Theamylase activity of the three bark beetles species was shown in the following sequence. D. armandi, I. acuminatus,and P. sinensis. The optimum temperature for proteinase activity of D. armandi larvae was 20 ℃ and that of adult was 25 C. The optimum temperature for amylase, lipase and cellulase activity of D. armahdi larvae equaled to that of D. armandi adult,40 ℃ 25 ℃ and 25 ℃ respectively. [Conclusion] Activity differences in protease,cellulase and amylase were significant among the three pine bark beetles, while lipase activity had no significant difference. And the four types of digestive enzyme activities of D. armandi adults and larvae changed with temperature.

关 键 词：华山松 小蠹虫 消化酶 酶活性 最适温度 

分 类 号：S791.241.07[农业科学—林木遗传育种]