放线菌SL-5链霉素耐药突变菌株的分离、活性筛选及发酵优化研究  

Studies on the isolation,activity screening and the fermentation optimization of streptomycin-resistance mutants of actinomycete SL-5

作　　者：沈玲[1] 郭正彦[1] 吴文君[1] 

机构地区：[1]西北农林科技大学农药研究所,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2008年第12期205-211,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家"973"项目"从天然生物源小分子发现农用新作用靶标机制及先导结构"(2003CB114404)

摘　　要：【目的】为新型抗生素的开发研究提供有效途径。【方法】对放线菌SL-5在链霉素不同浓度下的耐药突变菌株进行分离和活性筛选,并采用单因素和正交试验对活性菌株进行发酵优化。【结果】从无活性放线菌SL-5的耐药菌株中得到1株活性突变菌株str-14,室内生测结果表明,其发酵液对蜡状芽孢杆菌(Bacillus cereus)的抑菌圈直径为17.5 mm。通过单因素和正交试验,确定该菌株的最佳摇瓶发酵培养基配方为:小米10 g/L,葡萄糖20g/L,酵母粉2 g/L,蛋白胨7 g/L,混合VB1.5 mg/L,NaCl 2.5 g/L,CaCO32.0 g/L。最佳培养条件为:初始pH为8.0,装液量70 mL(250 mL三角瓶),180 r/min培养7 d。【结论】通过引入链霉素耐药性突变获得抑菌活性物质,是创制新型抗生素的一条可行之路。[Objective] The studies were carried out to provide an efficient way of discovering novel antibiotics. [Method] Mutants were isolated at different streptomycin concentrations from wild strain SL-5 and under bioactivity screening,then the fermentation medium optimization of the active mutant was studied by the single-factor and orthogonal test. [Result] Strain str -14 was obtained from the streptomycin-resistance mutants of inactive strain SL-5, and the result of bioassay in vitro showed that the fermentation broth of str-14 exhibited obvious inhibition circle of 17.5 mm against （Bacillus cereus）. The results of single-factor and orthogonal test showed that the optimum fermentation of strain str -14 for producing the most effective fermentation product were Millet 10 g/L, Glucose 20 g/L, Yeast extract 2 g/L, Peptone 7 g/L,Compound vitamins B 1.5 mg/L,NaC1 2.5 g/L,CaCOa 2.0 g/L. The optimum conditions were initial pH of 8.0,media volume 70 mL,shaking at 180 r/min for 7 days. [Conclusion] It is a feasible way for the discovery of new antibiotics to find active substance by inducing streptomycin-resistance mutation.

关 键 词：放线菌 链霉素耐药突变菌株 分离 发酵优化 

分 类 号：S859.796[农业科学—临床兽医学]