瑞香狼毒茎叶糖类成分的定性与定量分析  

Qualitative and quantitative analysis of saccharide compositions in the leaf and stem of Stellera chamaejasme L

作　　者：杨国栋[1] 宋晓平[1] 华彩丽[1] 付明哲[1] 杨向颖[1] 程飞[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2008年第12期212-216,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(30571402)

摘　　要：【目的】对瑞香狼毒茎叶中糖类成分进行定性定量分析。【方法】采用薄层色谱法,以氯仿-冰醋酸-蒸馏水混合液(V(氯仿)∶V(冰醋酸)∶V(蒸馏水)=18∶21∶3)为展开剂,苯胺-二苯胺磷酸丙酮溶液为显色剂,对瑞香狼毒茎叶中的可溶性糖进行定性检测。采用苯酚-硫酸比色法,以葡萄糖标准液制作标准曲线,对瑞香狼毒茎叶中可溶性糖和总糖含量进行测定。【结果】瑞香狼毒茎叶提取液的薄层色谱显示2个斑点,Rf值分别为0.457,0.519,分别与蔗糖、葡萄糖对照品的Rf值一致。葡萄糖标准液在490 nm处有最大吸收峰,其标准曲线方程为Y=0.007 8X-0.016(Y为490 nm处吸光度,X为葡萄糖标准液含量),相关系数r=0.998 6。采用苯酚-硫酸比色法测定瑞香狼毒茎叶中的糖含量可靠性高。【结论】瑞香狼毒茎叶中的可溶性糖主要为葡萄糖和蔗糖,可溶性糖和总糖含量分别为34.2和77.8 g/kg。[Objective] The total sugar and soluble sugar in the leaf and stem of Stellera chamaejasme L was investigated. [Method] The soluble sugar was detected by thin layer chromatography（TLC）. The plate was developed with chloroform-glacial acetic acid-distilled water（V（chlorlform） . V（glacial acetieacid） ： V（distilled water）=18 ： 21 ： 3）. The spots were located by exposing the plate to the solution of aniline,diphenylamine,phosphoric acid and acetone. The content of sugar was determined with phenol-sulfuric acid method and by reference to glucose. [Result] There were two spots in the plate, the Ri values were 0. 457 and 0. 519 respectively,which accorded with that of glucose and sucrose. The glucose standard liquid had the highest absorptive peak at 490 nm wavelength and the regression equation was A = 0. 007 8C- 0. 016 and Correlation coefficient r=0. 998 6. The average recoveries and relative standard deviation were 98.97% and 1. 662% respectively. The contents of soluble sugar and total sugar in the leaf and stem of Stellera chamaejasrne L detected by phenol-sulfuric acid method were credible. [Conclusion] The main compositions of soluble sugar in the leaf and stem of Stellera chamaejasme L were glucose and sucrose,and the contents of soluble sugar and total sugar in the leaf and stern of Stellera charnaejasme L were 34. 2 g/kg and 77.8 g/kg respectively.

关 键 词：瑞香狼毒 茎叶 糖类成分 苯酚-硫酸法 

分 类 号：S859.4[农业科学—临床兽医学]