紫花丹参和白花丹参不同部位有效成分的分布特征  

Distribution traits of bioactives in different parts of Salvia miltiorrhiza Bunge. and Salvia miltiorrhiza Bunge.f.alba

作　　者：杭亮[1] 王俊儒[2] 杨东风[1] 舒志明[1] 梁宗锁[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100 [2]西北农林科技大学理学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2008年第12期217-222,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：2005年度教育部新世纪优秀人才支持计划(NCET-05-0855);2005年度西北农林科技大学青年学术骨干支持计划

摘　　要：【目的】探讨紫花丹参、白花丹参中根、茎、叶、花等部位有效成分的分布特征。【方法】采用紫外分光光度法、NaNO2-AlCl3-NaOH法和Folin-Ciocalteu比色法测定紫花丹参、白花丹参中根、茎、叶、花等部位的总丹参酮、总黄酮、总酚酸含量,采用高效液相色谱法(HPLC)测定丹参酮ⅡA、丹参素钠、原儿茶醛和丹酚酸B的含量。【结果】总丹参酮、总黄酮、总酚酸含量在紫花丹参和白花丹参的根和叶中均较高,而在茎中较低。其中紫花丹参叶部总丹总丹参酮、总黄酮、总酚酸含量分别比茎部高2.5倍、3.8倍和3.5倍。白花丹参叶部这3类成分含量分别比茎部高2.4倍、1.8倍和1.9倍。紫花丹参和白花丹参的地上部均未检测出丹参酮ⅡA;但含有与根部相同的丹参素、原儿茶醛和丹酚酸B等3种水溶性成分,且在叶中含量较高。【结论】除根以外,丹参叶部各活性成分的含量均较高,可作为提取丹酚酸等的原料。此外,白花丹参多数部位有效成分含量高于紫花丹参,是值得进一步开发利用的种质资源。[Objective] The research studied the distribution traits of bioactives in different parts- root, stem,leaf, flower of Salvia miltiorrhiza Bunge. and Salvia miltiorrhiza Bunge. f. alba. [Method] Ultraviolet-visible spectrophotometric method was used to determine the contents of total tanshinones, total flavonoids and total phenolic acids in the root,the stem,the leaf and the flower of S. miltiorrhiza Bunge and S. rniltiorrhiza Bunge. f. alba. And HPLC was used to determine the contents of tanshinone 11 A, sodium danshensu, protocatechuic aldehyde, salvianolic acid B. [Result] The contents of total tanshinones, total flavonoids and total phenolic acids were higher in the root and the leaf of the two varieties of S. miltiorrhiza, lower in the stem. The contents of total tanshinones, total flavonoids and total phenolic acids in the leaf of S. miltiorrhiza Bunge were 2.5 times, 3.8 times,and 3.5 times higher than that in the stem respectively. The contents of the three type biouctives in the leaf of Salvia miltiorrhiza Bunge. f. alba were 2.4 times, 1.8 times and 1.9 times higher than that in the stem respectively. The analysis results by HPLC showed that there was no tanshinone ⅡA in the aerial parts of the two varieties of S. miltiorrhiza, but the aerial parts contained the same water-soluble constituents as in the roots,and higher contents occured in the leaf. [Conclusion] The leaf has higher contents of bioactive components among the aerial parts, therefore, hesides the root, the leaf can be the resource of obtaining bioactives such as salvianolic acids. In addition, most parts of S. miltiorrhiza Bunge. f. alba have higher contents of bioactives than that in S. miltiorrhiza Bunge., suggesting that S. miltiorrhiza Bunge. f. alba is a kind of germplasm resource to be exploited.

关 键 词：紫花丹参 白花丹参 不同部位 有效成分 

分 类 号：Q946.8[生物学—植物学] S567.53[农业科学—中草药栽培]