人脐血间充质干细胞分离、培养及向成骨细胞分化的实验研究  

Study on isolation cultured of human cord blood mesenchymal stem cells and its induction differentiation into osteoblasts

作　　者：陶艳玲[1] 张颢[2] 

机构地区：[1]广西壮族自治区人民医院儿科,广西南宁530021 [2]广西壮族自治区人民医院血液科,广西南宁530021

出　　处：《临床荟萃》2008年第23期1695-1697,共3页Clinical Focus

摘　　要：目的探讨脐血间充质干细胞(MSCs)的分离培养及向成骨细胞的分化潜能。方法从足月儿脐血中获得间充质干细胞,体外培养传代,流式细胞检测细胞表面及抗原细胞周期;1.0×10-8mol/L地塞米松,2.0×10-4mol/L抗坏血酸,7.0×10-3mol/Lβ-磷酸甘油的IMDM培养基对MSCs进行诱导向脂肪细胞分化,von-Kossa染色鉴定。结果成功建立了脐血间充质干细胞分离及培养扩增的方法;流式细胞仪检测结果显示,贴壁细胞均表达CD105、CD29和CD44,不表达造血细胞表型CD34、CD45和内皮细胞表型CD31;经脂肪培养液诱导后细胞间可见黑色矿化物沉积阳性。结论脐血间充质干细胞能进行体外分离培养,并能诱导分化为成骨细胞。Objective To establish a method to separate mesenchymal stem cells（MSCs） from human umbilical cord blood（UCB） ,culture UCB-MSCs in vitro,and study its biological characteristics. Methods MSCs were seperated from human umbilical cord blood and cultured in vitro,surface antigens were detected with flow cytometry;MSCs were induced to differentiate into osteoblasts in induction medium containing 1.0 × 10^-8 mol/L desamethasone, 2.0 × 10^-4 mol/L ascorbic acid and 7.0× 10^-3 mol/L β-sodium glycerophosphate. Then differentiated cells were identified by von- Kossa stain. Results The method of culture and expansion of UCB-MSC was established in vitro. Adhesive cells were all positive for MSCs-related antigens, such as CD105, CD29 and CD44, but negative for CD34, CD45 and CD31. The colony cells differentiating into osteoblasts contained black mineralized deposition by von-Kossa stain on the 14th day in adipose medium. Conclusion The cultured MSCs,with the biological characteristics of MSCs, can be amplified easily, passaged and differentiated into osteoblasts in vitro.

关 键 词：间质干细胞 胎血 脐带 细胞培养技术 

分 类 号：R318[医药卫生—生物医学工程]