HPLC测定藏边大黄中的藏黄苷A  

Content determination of piceatannol-4'-O-β-D-glucopyranoside in Rheum emodii

作　　者：卢敬光[1] 王曙[1] 严晓梁[2] 马静[1] 何俊[1] 刘傲镭[1] 吴启秀[1] 

机构地区：[1]四川大学华西药学院,四川成都610041 [2]四川省人民医院,四川成都610072

出　　处：《华西药学杂志》2008年第6期704-705,共2页West China Journal of Pharmaceutical Sciences

摘　　要：目的测定不同来源藏边大黄中的藏黄苷A。方法采用HPLC法,色谱柱为Kromasil C18、Shim-pack C18(250mm×4.6mm,5μm),检测波长为320nm,流动相为乙腈-水(15:85),流速为1.0ml·min-1,柱温为30℃。结果藏黄苷A0.17～17.00μg与峰面积呈良好的线性关系(r=0.9999,n=6),平均加样回收率为100.30%,RSD=1.0%(n=6);5份藏边大黄中藏黄苷A的含量均大于7.5%。结论所建方法简单、稳定,藏黄苷A的含量可作为藏边大黄的品质评价依据。OBJECTIVE To detect piceatannol -4＇ - O - β - D - glucopyranoside （PGP） in R. emodii from different origins. METHODS An HPLC method was used. The column was Kromasil C18 or Shim -pack C18 （250 mm ×4. 6 mm,5μm）. The UV detection wavelength was 320 nm The mobile phase consisted of acetonitrile -water（ 15 ： 85 ） and the flow rate was 1.0 ml. min^-1 with column temperature of 30℃. RESULTS The calibration curve of PGP was linear over the ranges of 0. 17 - 17 μg （ r = 0. 9999, n = 6 ）. The average recovery rate was 100. 30% with RSD of 1.0% （n =6）. PGP in R. ernodii was more than 7. 5% from five different origins. CONCLUSION This method is simple and stable. The content of PGP can offer scientific assurance for quality valuation for R. emodii.

关 键 词：HPLC 藏边大黄 藏黄苷A 芪类化合物 

分 类 号：R917[医药卫生—药物分析学]