鸭、鹅Gal-6的克隆与序列分析  

Cloning and Sequence Analysis of Gal-6 Gene of Duck and Goose

作　　者：江龙海[1] 祁克宗[1] 彭开松[1] 高恒[1] 朱枫桥[1] 

机构地区：[1]安徽农业大学动物科技学院,安徽合肥230036

出　　处：《中国家禽》2008年第22期21-24,共4页China Poultry

基　　金：国家"863"计划(2006AA10Z320);国家自然科学基金(30800811);安徽省"十五"科技重大攻关项目(0400303041);安徽省优秀青年基金(04041042)

摘　　要：根据GenBank公布的鸡β-防御素Gal-6基因序列设计2对引物,应用反转录-聚合酶链式反应技术,从鸭、鹅不同组织中扩增了β-防御素基因片段,大小为250bp。选取鸭16个组织和鹅的肝脏检测其表达情况,结果表明,除在肾、皮肤、法氏囊外,其他组织均检测为阳性。经筛选获得重组质粒并测序,从重组质粒中扩增出成熟肽,大小约150bp。克隆的多肽由67个氨基酸组成,分别是20个氨基酸残基的信号肽、5个氨基酸的原片段、42个氨基酸的成熟肽。BLAST分析表明,鸭基因序列与基因库中已公布的序列相差1个碱基,鹅基因序列则完全相同。According to the similar gene sequence of birds in GenBank,two pairs of primers were designed. Then Gal-6 fragment was amplified by reverse transeription-polymerase chain reaction from different part of duck and goose. Results showed that the specific bands of 250 bp were found. Except kidney,skin and bursa of Fabricius,there was positive expression in other 14 samples of duck and liver of goose. The recombinant plasmid was sequenced. The mature peptide sequence was 150 bp. The results showed that the cDNA fragment coded 67 amino acid residues,which comprised of signal peptide with 20 amino acid residues,propriece peptide with 5 amino acid residues,and mature peptide with 42 amino acid residues. BLAST analysis showed that there was a base difference in gene sequence between duck and chicken,and the gene sequences of goose was completely homology to chicken.

关 键 词：鸭 鹅 Gal-6 克隆 序列分析 

分 类 号：S858.33[农业科学—临床兽医学] Q78[农业科学—兽医学]