4-PU和6-BA对‘汕油523’花生上胚轴愈伤组织诱导及不定芽发生的影响  

Effects of 4-PU and 6-BA on Formation of Calli and Adventitious Buds in Epicotyl Explants of Arachis hypogaea

作　　者：覃铭[1,2] 刘璨[1] 李玲[1] 

机构地区：[1]华南师范大学生命科学学院广东省植物发育生物工程重点实验室,广东广州510631 [2]北京师范大学珠海分校工程技术学院,广东珠海519085

出　　处：《亚热带植物科学》2008年第4期15-17,28,共4页Subtropical Plant Science

基　　金：广东省科技计划项目(2006B20101002)资助

摘　　要：以‘汕油523’花生上胚轴为外植体,研究细胞分裂素4-PU和6-BA对愈伤组织诱导及不定芽发生的效应。结果表明,当MS培养基含有4-PU0.1mg/L和6-BA4mg/L培育20d时,愈伤组织形成率为100%;0.1mg/L4-PU与适宜浓度的6-BA(1～4mg/L)共同作用,促进上胚轴不定芽发生,不定芽形成与芽伸长的适宜培养基配方分别为MS+4-PU0.1mg/L+6-BA2mg/L和MS+4-PU0.1mg/L+6-BA4mg/L;0.5mg/L4-PU对不定芽出芽率、芽数与长度的作用均大于其与不同浓度6-BA混合作用的效果。The effects of 4-PU and 6-BA on formations of the calli and adventitious buds from epicotyl explants in peanut were studied in the experiment. Cultured in the MS medium contained 0.1mg/L 4-PU and 6-BA 4mg/L for 20 days, the formation rate of calli could reach 100%. 0.1mg/L 4-PU combined with 1-4mg/L6-BA in MS medium promoted the adventitious buds formation. The optimal medium for adventitious bud formation and shoot elongation were MS ＋0.1 mg/L 4-PU ＋ 2mg/L 6-BA and MS＋0.1mg/L 4-PU＋4mg/L BA, respectively. The percentage of adventitious bud formation, number and length of buds of peanut grown in the MS medium contained only 0.5mg/L 4-PU were higher than those grown in the MS medium contained 0.5mg/L 4-PU＋6-BA.

关 键 词：4-PU 6-BA 花生 愈伤组织 不定芽 

分 类 号：Q943.1[生物学—植物学] S565.2[农业科学—作物学]