Factors affecting mito-nuclear codon usage interactions in the OXPHOS system of Drosophila melanogaster  

作　　者：Zheng Sun Liang Ma Robert W. Murphy Xiansheng Zhang Dawei Huang 

机构地区：[1]College of Plant Protection, Shandong Agricultural University, Tai＇an 271018, China [2]Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China [3]Department of Natural History, Royal Ontario Museum, 100 Queen＇s Park, Toronto, ON M5S 2C6 Canada [4]College of Life Sciences, Shandong Agricultural University, Tai＇an 271018, China

出　　处：《Journal of Genetics and Genomics》2008年第12期729-735,共7页遗传学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No.30770302 and 30570970);the Program of Ministry of Science and Technology of the Republic of China (No.2006FY110500);partially by the National Science Fund for Fostering Talents in Basic Research (NSFC-J0630964/J0109)

摘　　要：Codon usage bias varies considerably among genomes and even within the genes of the same genome. In eukaryotic organisms, energy production in the form of oxidative phosphorylation （OXPHOS） is the only process under control of both nuclear and mitochondrial genomes. Although factors affecting codon usage in a single genome have been studied, this has not occurred when both interactional genomes are involved. Consequently, we investigated whether or not other factors influence codon usage of coevolved genes. We used Drosophila melanogaster as a model organism. Our χ^2 test on the number of codons of nuclear and mitochondrial genes involved in the OXPHOS system was significantly different （χ^2= 7945.16, P 〈 0.01）. A plot of effective number of codons against GC3s content of nuclear genes showed that few genes lie on the expected curve, indicating that codon usage was random. Correspondence analysis indicated a significant correlation between axis 1 and codon adaptation index （R = 0.947, P 〈 0.01） in every nuclear gene sequence. Thus, codon usage bias of nuclear genes appeared to be affected by translational selection. Correlation between axis 1 coordinates and GC content （R = 0.814, P 〈 0.01） indicated that the codon usage of nuclear genes was also affected by GC composition. Analysis of mitochondrial genes did not reveal a significant correlation between axis 1 and any parameter. Statistical analyses indicated that codon usages of both nDNA and mtDNA were subjected to context-dependent mutations.Codon usage bias varies considerably among genomes and even within the genes of the same genome. In eukaryotic organisms, energy production in the form of oxidative phosphorylation （OXPHOS） is the only process under control of both nuclear and mitochondrial genomes. Although factors affecting codon usage in a single genome have been studied, this has not occurred when both interactional genomes are involved. Consequently, we investigated whether or not other factors influence codon usage of coevolved genes. We used Drosophila melanogaster as a model organism. Our χ^2 test on the number of codons of nuclear and mitochondrial genes involved in the OXPHOS system was significantly different （χ^2= 7945.16, P 〈 0.01）. A plot of effective number of codons against GC3s content of nuclear genes showed that few genes lie on the expected curve, indicating that codon usage was random. Correspondence analysis indicated a significant correlation between axis 1 and codon adaptation index （R = 0.947, P 〈 0.01） in every nuclear gene sequence. Thus, codon usage bias of nuclear genes appeared to be affected by translational selection. Correlation between axis 1 coordinates and GC content （R = 0.814, P 〈 0.01） indicated that the codon usage of nuclear genes was also affected by GC composition. Analysis of mitochondrial genes did not reveal a significant correlation between axis 1 and any parameter. Statistical analyses indicated that codon usages of both nDNA and mtDNA were subjected to context-dependent mutations.

关 键 词：Drosophila melanogaster oxidative phosphorylation NDNA MTDNA codon usage 

分 类 号：Q244[生物学—细胞生物学] Q969.462.2