三氧化二砷对MRL/lpr自发性狼疮小鼠脾脏单个核细胞DNMT1及CD_(11a)表达的影响  

Effects of arsenic trioxide on the expression of DNA methyltransferase 1 and CD(11a) genes in splenic mononuclear cells of spontaneous lupus nephritis MRL/lpr mice

作　　者：王红[1] 孙莉[2] 陈培荣[2] 周艳[2] 朱小春[2] 

机构地区：[1]温州医学院附属第二医院肾病风湿免疫科,325027 [2]温州医学院附属第一医院风湿免疫科

出　　处：《浙江医学》2008年第11期1191-1192,1236,共3页Zhejiang Medical Journal

基　　金：浙江省中医药科技计划项目(Y200605339)

摘　　要：目的探讨三氧化二砷(ATO)对MRL/lpr自发性狼疮小鼠脾脏单个核细胞(SMC)中DNA甲基转移酶1(DNMT1)和黏附分子淋巴细胞功能相关抗原-1(LFA-1)的亚基CD11a表达的影响及其可能作用机制。方法分离小鼠SMC,采用20g/ml植物血凝素和1000IU/ml白介素-2体外诱导培养SMC 48h后,随机分为空白组、ATO组和5-氮胞苷(5-Az)组,继续培养24h,采用RT-PCR法检测两组小鼠SMC中DNMT1及CD11a基因转录水平的表达情况。结果MRL/lpr小鼠ATO组中DNMT1-mRNA的灰度比值较5-Az组增高(P<0.05),MRL/lpr小鼠ATO组中CD11a-mRNA的灰度比值较5-Az组降低(P<0.05)。结论ATO治疗系统性红斑狼疮的作用机制可能与其能逆转低甲基化状态有关。Objective To investigate the effect of arsenic trioxide （ATO） on the expression of DNA methyltransferasel （DNMT1）mRNA and CD11a mRNA in splenic mononuclear cells （SMNC）of spontaneous lupus nephritis MRl_/Ipr mice. Methods Isolated SMNC of MRL/Ipr and C57BL mice were induced by PHA 20g/ml ＋ IL-2 10001U/rot for 48 h, and the cultured cells were randomly divided into control group, ATO group, 5-AZ group. Each group was incubated in vitro for 24h and RNA was extracted. The expression of DNMT1 and CD11a was determined by RT-PCR. Results The gray scale value of DNMTI-mRNA in ATO group was significantly higher than that in 5-Az group （P 〈 0.05）and the gray scale value of CD11a-mRNA in ATO group was significantly lower than that in 5-Az group （ P〈 0.05）. Conclusion The therapeutic effect of ATO for SLE may be associated with reversed low DNA methylation.

关 键 词：三氧化二砷 5-氮胞苷 系统性红斑狼疮 DNA甲基转移酶1 CD11A 

分 类 号：R593.241[医药卫生—内科学]