参附注射液对失血性休克大鼠血清及人脐静脉内皮细胞TM和EPCR表达的影响  

Effects of Shenfu injection on thrombomodul and endothelial cell protein C receptor levels in serum of hemorrhagic shock rats and human umbilical vein endothelial cells

作　　者：张近波[1] 潘景业[1] 李智强[1] 陈洁[1] 姚震亚[1] 王晓蓉[1] 

机构地区：[1]温州医学院附属第一医院ICU,325000

出　　处：《浙江医学》2008年第11期1193-1195,共3页Zhejiang Medical Journal

基　　金：浙江省教育厅基金资助项目(20041056)

摘　　要：目的探讨参附注射液对失血性休克大鼠血清和人脐静脉内皮细胞(HUVEC)中血栓调节蛋白(TM)、内皮细胞蛋白C受体(EPCR)表达的影响。方法将50只SD大鼠随机分为正常对照组、假休克组、失血性休克组、林格注射液治疗组(林格组)、林格注射液加参附注射液治疗组(参附组),分别检测各组可溶性TM(sTM)、可溶性EPCR(sEPCR)水平及TM、EPCR mRNA和蛋白表达水平。结果与假休克组比较,休克组和林格组sTM、sEPCR及TM、EPCR mRNA含量均增高(P<0.05),TM和EPCR蛋白含量均降低(P<0.05)。与林格组比较,参附组sTM、sEPCR含量和TM、EPCR mRNA含量均下降(P<0.05),TM和EPCR蛋白含量均升高(P<0.05)。结论参附注射液可从基因、蛋白水平影响人血清和HUVEC中TM和EPCR的表达,从而阻止失血性休克的进展。Objective To investigate the effects of Shenfu injection on thrombomodul （TM） and endothelial cell protein C receptor （EPCR） levels in serum of hemorrhagic shock rats and human umbilical vein endothelial cells. Methods Fifty rats were randomly divided into five groups： normal control group, sham shock group, hemorrhagic shock group, Linger-treated group and Shenfu-treated group. The soluble thrombomodulin （sTM） , endothelial protein C receptor （ sEPCR）,TM, EPCRmRNA and protein were detected by enzyme linked lmmunosorbent assay （ELISA）, reverse transcription polymerase chain reaction （RT-PCR）, western blotting （WB）, respectively. Results The mean levels of sTM, sEPCR and the expression of TM, EPCR mRNA in hemorrhagic shock and Linger-treated group was significantly higher than that in sham shock （ P〈 0.05）,while the level of protein was lower than that in sham shock （P〈0.05）. The mean levels of sTM, sEPCR and expression of TM, EPCR mRNA in Shenfu-treated group was significantly lower than that in Linger-treated group （ P〈 0.05）,while the level of protein was higher than that in Linger-treated group （P〈0.05）. Conclusion Results suggest that Shenfu injection can influence TM and EPCR mRNA and protein expression, therefore it might be effective in prevention of development of hemorrhagic shock.

关 键 词：失血性休克 参附注射液 血栓调节蛋白 内皮细胞蛋白C受体 内皮细胞 

分 类 号：R285.5[医药卫生—中药学]