冬虫夏草对大鼠肠黏膜屏障免疫功能影响的研究  

Effects of Cordyceps sinensis on immune function of intestine mucosal barrier in rat

作　　者：顾国胜[1] 任建安[1] 李宁[1] 黎介寿[1] 

机构地区：[1]南京大学医学院临床学院(南京军区南京总医院)解放军普通外科研究所,江苏南京210002

出　　处：《肠外与肠内营养》2008年第6期326-328,共3页Parenteral & Enteral Nutrition

基　　金：国家自然科学基金面上项目(30571797);江苏省自然科学基金重大项目(BK2006719)

摘　　要：目的:探讨冬虫夏草对T淋巴细胞亚群及小肠黏膜屏障免疫功能的影响。方法:将大鼠分为对照组和实验组。实验组大鼠给予冬虫夏草5g/(kg·d)灌胃;对照组给予等量的等渗盐水灌胃,两组均自由进食、饮水。于灌胃第4、7和10天分别抽取8只大鼠的血,用流式细胞仪检测外周血液中CD3+、CD4+、CD8+的百分率,计算CD4+/CD8+比例。同时取大鼠小肠黏膜,行常规病理计数上皮内淋巴细胞(IEL)的比例,用免疫组化染色检测CD4+、CD8+,计算CD4+/CD8+的比例。结果:实验组大鼠第4天外周血中CD4+淋巴细胞即有明显提高,CD4+/CD8+的比例明显升高,至第7和第10天变化更为明显。肠黏膜上皮内淋巴细胞的数量和CD4+/CD8+比例的变化也较明显,差异有显著性意义(P<0.05)。结论:冬虫夏草可使正常大鼠外周血T淋巴细胞含量增加,同时可增强小肠黏膜的免疫屏障功能。Objective：To study the effects of Cordyceps sinensis on T lymphocyte subsets in peripheral blood and the immune function of intestine mueosal barrier in rat. Methods ： 48 rats were randomly di- vided into two groups： normal control group and Cordyeeps sinensis treated group [ 5 g/（kg· d）, ig]. The peripheral blood lymphocyte subsets percentages and the CD4 ＋/CD8 ＋ ratio was measured by the flow cytometry. The intraepithelial lymphocyte （IEL） and CD4 ＋/CD8＋ ratio of intestinal mucosa were studied by using image analysis and immunohistoehemieal technique. Results： Compared with normal control group, the percentages of CD4 ＋ T lymphocyte and CD4＋/CD8 ＋ ratio in the peripheral blood in Cordyceps sinensis treated rats were siginificantly increased （P 〈 0.05 ）. The percentages of IEL and CD4 ＋/CD8 ＋ ratio of intestinal mucosa showed obvious difference between the control and the treated groups （P 〈 0.05 ）. Conclusion ： Cordyceps sinensis can increase the percentages of the T lymphocyte in the peripheral blood in rat, and enhance immune function of intestine mucosal barrier.

关 键 词：冬虫夏草 T淋巴细胞亚群 上皮内淋巴细胞 流式细胞术 

分 类 号：R285.5[医药卫生—中药学]