HeLa、HEK293、SH-SY5Y细胞中的Tau蛋白  

Localization of Tau Protein in SH-SY5Y, HeLa and HEK293 Cells

作　　者：盛之玲[1,2,3] 曲梅花[1] 何海进[1] 赫荣乔[1] 

机构地区：[1]中国科学院生物物理研究所,北京100101 [2]中国科学院研究生院,北京100049 [3]郑州大学医学院,郑州450052

出　　处：《生物化学与生物物理进展》2008年第12期1364-1370,共7页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金(90206041,30570536);国家重点基础研究发展计划(973)(2006CB500703);中国科学院知识创新项目(KSCX2-YW-R-119)资助~~

摘　　要：通过间接免疫荧光测定了HeLa、HEK-293、SH-SY5Y细胞内Tau蛋白的分布,观察到在细胞间期单克隆抗体Tau-1的荧光信号分布于细胞质和胞核中.特别是HeLa细胞,其胞核内具有相对较高的Tau蛋白免疫荧光信号.通过分离SH-SY5Y的细胞核,更为清楚地显示了Tau蛋白在细胞核中的分布,并且免疫荧光信号与DNA的Hoechst33258染色信号相重合.Western blotting的测定结果进一步证明了SH-SY5Y细胞的胞质和胞核中均含有Tau蛋白的不同异构体.以上结果提示,Tau蛋白不仅存在于神经、肌肉等细胞内,也存在于肿瘤细胞系,并且分布于间期的胞核中.Using the Tau-1 （or Tau-13） monoclonal antibody, the distribution of endogenetic Tau in the SH-SY5Y cells, HEK-293 cells and HeLa cells have been observed, and the localization of the indirect immunofluorescent signals in both cytoplasm and nuclei in the interphase have been found. In particular, the fluorescent signals were significantly strong in HeLa nuclei. During cell division （metaphase and anaphase）, few Tau-1 signals were observed in the nuclei under the same conditions. To confirm the nucleoplasm localization of Tau, the nuclei of SH-SY5Y cells were isolated and it was observed that Tau-I signals were co-localized with Hoechst33258 （blue） in nucleoplasm using double immunofluorescence. Western-blotting also exhibited tau protein isoforms localized in both cytoplasm and nucleoplasm. It indicates that Tau protein is localized in tumor cells, and also in their nucleoplasm.

关 键 词：TAU蛋白 神经Tau 肿瘤细胞 细胞核 Tau与DNA相互作用 

分 类 号：Q5[生物学—生物化学] Q6