人参皂甙Rb1对老年性痴呆模型大鼠顶叶皮质β-分泌酶及早老蛋白-1表达的影响  

Effect of ginsenoside Rb1 on expression of β-secretase and presenilin-1 in the parietal lobe of Alzheimer disease-like rat

作　　者：方欣[1] 杨吉平[1] 赖红[1] 

机构地区：[1]中国医科大学基础医学院解剖学教研室,沈阳110001

出　　处：《解剖学杂志》2008年第6期812-815,共4页Chinese Journal of Anatomy

基　　金：辽宁省自然科学基金(20062088)

摘　　要：目的:探讨人参皂甙Rb1对老年性痴呆模型大鼠顶叶皮质β-分泌酶及早老蛋白-1(PS-1)表达水平的影响。方法:30只SD大鼠随机分为对照组、模型组和治疗组。模型组大鼠胃饲氯化铝200mg·kg-1.d-1,同时腹腔注射D-半乳糖60mg·kg-1.d-1;治疗组大鼠于造模结束后,腹腔注射人参皂甙Rb110μg·kg-1.d-1。采用免疫组织化学方法和图像分析技术检测β-分泌酶及PS-1的表达,通过Morris水迷宫测试观察大鼠学习记忆能力的改变。结果:与对照组比较,模型组β-分泌酶及PS-1的表达明显增多,学习记忆能力明显下降;与模型组相比,治疗组β-分泌酶及PS-1的表达明显减少,学习记忆能力在第2天及第3天显著提高。结论:人参皂甙Rb1可以抑制β-分泌酶及PS-1的表达,对老年性痴呆模型大鼠有一定的防治作用。Objective： To investigate the effect of ginsenoside Rb1 on the expression of β-seeretase and presenilin-1 （PS-1） in the parietal lobe of Alzheimer disease-like rats. Methods： Thirty SD rats were randomly divided into a control group, a model group and a treatment group. The model rats were established with administration of I）-galactose 60 mg· kg-1· d- 1 and aluminum chloride 200 mg·kg -1 · d-1 for 2 months, the control group were given the same dose of sodium chloride, and treatment group were given ginsenoside Rb1 10μg · kg-1· d -1 for 4 weeks after the model rats were established. The expressions of β-seeretase and PS-1 in the parietal lobe were detected by immunohistochemistry with image analysis. Morris test was used to determine the ability of learning and memory. Results： Compared with the control group, the levels of β-secretase and PS-1 were significantly higher in the model group （P〈0.05）, and learning and recognition ability in this group was obviously worse （P〈0.05） ; Compared with the moctel group, the levels of β-secretase and PS-1 were significantly low- er in the treatment group （P〈0.05）, and learning and recognition ability in this group was better. The learning and recog- nition ability was obviously improved on the second and the third day （P〈0.05）. Conclusion： Ginsenoside Rbl has preven- tive and therapeutic effect on Alzheimer disease-like rats, via inhibiting the expression of β-seeretase and PS-1.

关 键 词：大脑皮质 Β-分泌酶 早老蛋白-1 人参皂甙RB1 阿尔茨海默病 

分 类 号：R285.5[医药卫生—中药学]