黄芪注射液对哮喘大鼠p38MAPK和Th1／Th2类细胞因子变化的影响  

Protective effects and mechanism of astragalus injection on asthmatic rats

作　　者：黄翠萍[1] 杨和平[1] 杨颖乔[1] 张珍祥[2] 徐永健[2] 

机构地区：[1]咸宁学院医学院内科,湖北咸宁437100 [2]华中科技大学同济医学院附属同济医院呼吸内科,湖北武汉430030

出　　处：《中国病理生理杂志》2008年第12期2386-2390,共5页Chinese Journal of Pathophysiology

基　　金：湖北省科技厅科研资助项目(No.2006ABA330)

摘　　要：目的:探讨黄芪注射液对哮喘大鼠气道炎症和Th1/Th2类细胞因子(IFN-γ/IL-4)变化的影响及其可能机制。方法:雄性SD大鼠40只随机分成5组,即正常对照组、哮喘模型组和黄芪低、中、高剂量干预组。分别采用酶联免疫吸附法(ELISA)、逆转录-聚合酶链反应(RT-PCR)和蛋白质印迹检测支气管肺泡灌洗液(BALF)中IL-4、IFN-γ含量、肺组织中IL-4 mRNA、IFN-γ mRNA表达和磷酸化p38MAPK的变化,并观察BALF中炎症细胞和肺组织病理学改变。结果:哮喘模型组大鼠BALF中炎症细胞计数、IL-4含量和肺组织中IL-4 mRNA、磷酸化p38MAPK表达水平升高,IFN-γ、IFN-γ mRNA水平降低,与正常对照组比较,显著差异(P<0.01)。黄芪低、中、高剂量干预组大鼠BALF中炎症细胞计数、IL-4含量和肺组织中IL-4 mRNA、磷酸化p38MAPK表达水平均明显降低,IFN-γ、IFN-γ mRNA水平明显上升,与哮喘模型组比较,均具有显著差异(P<0.01)。黄芪处理能明显减轻哮喘大鼠肺组织病理学改变,但黄芪低、中、高剂量干预组之间比较,无显著差异(P>0.05)。肺组织磷酸化p38MAPK的表达与EOS计数、IL-4、IL-4 mRNA之间分别呈显著正相关(r=0.63,r=0.69,r=0.71,P<0.01),与IFN-γ和IFN-γmRNA之间分别呈显著负相关(r=-0.65,r=-0.68,P<0.01)。结论:p38MAPK可能参与了支气管哮喘的发病过程。黄芪注射液对哮喘大鼠具有保护作用,其机制可能与抑制p38MAPK磷酸化、纠正IFN-γ/IL-4平衡失调、减轻炎症细胞浸润有关。AIM： To explore the protective effects and mechanism of astragalus injection on asthmatic rats. METHODS ： OVA was injected intrapefitoneally and inhaled to produce the asthmatic model. Forty rats were randomly divided into five groups： control group, asthma group and astragalus groups of high, medium and low dose. The concentrations of IL-4, IFN-γ/ in BALF, the expression of IL-4 mRNA, IFN-γ/mRNA and phospho- p38 MAPK in lung tissues were respectively measured by ELISA, RT - PCR and Western blotting. The number of inflammatory cells in BALF and histropathology changes were observed. RESULTS ： In asthmatic group, the number of inflammatory cells and the concentrations of IL - 4 in BALF and the expression of IL - 4 mRNA, phospho - p38 MAPK in lung tissue were higher, but IFN-γ/ and IFN-γ/mRNA were lower than those in normal control rats （P 〈0. 01 ）. In astragalus group, the number of inflammatory cells, the concentrations of IL- 4 in BALF and the expression of IL- 4 mRNA, phospho- p38 MAPK in lung tissue were lower, hut IFN -γ/and IFN -γ mRNA were higher than those in normal control rats （P 〈 0. 01 ） , and histropathology damage was alleviated significantly. The efficacies in the astragalus groups of high, medium and low dose were similar, which no significant difference was observed among them. There were positive correlations between the expression of phospho - p38 MAPK and the number of eosinophil, the concentration of IL - 4, IL - 4 mRNA （ r = 0. 63, r = 0. 69, r = 0. 71,P 〈0. 01 ）, and negative correlations between the expression of phospho - p38 MAPK and IFN -γ/ and IFN - 3, mRNA （r = -0. 65, r = -0. 68, P 〈 0. O1 ）. CONCLUSION： p38 MAPK may play a role in pathological process of asthma. Astragalus effectively treats asthma by inhibiting the expression of phospho - p38 MAPK, correcting the inbalance of IFN -γ/IL - 4 and decreasing the number of inflammatory cells.

关 键 词：P38MAP激酶 哮喘 气道炎症 细胞因子类 黄芪 

分 类 号：R56[医药卫生—呼吸系统]