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作 者:向发云[1] 曾祥国[1] 冯小明[1] 吴金平[1] 吴润玲[1] 宋志红[1] 顾玉成[1]
机构地区:[1]湖北省农业科学院经济作物研究所,湖北武汉430064
出 处:《安徽农学通报》2008年第23期35-36,共2页Anhui Agricultural Science Bulletin
基 金:湖北省农业科技成果转化项目(2006050549);湖北省农业科技创新中心资助项目(2007-620-001-03)
摘 要:草莓花药培养再生率较低,其主要限制因素是花药愈伤的分化能力太低。在已有的研究基础上,对草莓花药培养中出现的黄绿色颗粒状、绿色致密、淡黄色蓬松这三种不同生理状态类型的愈伤组织进行研究,比较了草莓花药不同类型愈伤的再生能力,以期进一步提高草莓花药培养力。There were three calli in the process of anther callus induction and sub - culture in strawberry. Type Ⅰ callus was slow, green, rannlarstate. Typ Ⅱ callus was flourish, fresh - whiteandswampy. Type Ⅲ ( embroenic ) callus was grow in fresh -yellow, and crisstate. Type Ⅰ callus was induced from F medium. Type Ⅱ callus was obtained from type Ⅰ callus cultured on MS medium. The quality of type Ⅱ callus was improved when type Ⅱ callus was sub - cultured on MS medium. We wish to improve the anther culture ability more.
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