包埋-脱水法常温和低温下保存盐生杜氏藻的研究  

Storage of Dunaliella salina by Encapsulation-dehydration under Normal Temperature and Low Temperature

作　　者：董光宇[1] 张恩栋[1] 王起华[1] 

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116029

出　　处：《安徽农学通报》2008年第23期37-39,共3页Anhui Agricultural Science Bulletin

基　　金：国家自然科学基金资助项目(No.30170099;No.30470184)

摘　　要：用包埋-脱水法在常温和低温保存盐生杜氏藻(Dunaliella salina),探讨了温度、光暗和含水量等因素对存活率的影响。结果表明:盐生杜氏藻在4℃下保存6个月后的最高存活率达到54.4%。保存后的藻细胞经过恢复培养后,其生长力可达到保存前的水平。包埋-脱水法操作简单,无需复杂设备,在藻类种质的中期保存中有广阔的应用前景。Dunaliella salina was preserved by encapsulation - dehydration under normal temperature and low temperature. The main factors influencing the algal viability, such as different temperature, illumination intensities, the water content of beads, cell density, the volume of sealed plastic packing and the outer material of sealed plastic packing, were studied. The results showed that alginate beads were stored for 6 months under normal temperature and low temperature. The algae viability reached about 54. 4 % at 4℃ （reduced light）. After preservation, the growth rates of algae could reach the normal level in the conditions suitable for recovery.

关 键 词：盐生杜氏藻 常温保存 低温保存 包埋-脱水法 

分 类 号：Q93[生物学—微生物学]