模拟微重力对大鼠睾丸中睾酮合成相关基因表达的影响  

Effects of Simulated Microgravity on Gene Expression Related to Testosterone Synthesis in Testis of Rats

作　　者：周艺[1] 朱宝安[1] 郭爱娟[1] 谭信[1] 

机构地区：[1]北京理工大学生命科学与技术学院航天生物与医学工程研究所,北京100081

出　　处：《航天医学与医学工程》2008年第6期461-464,共4页Space Medicine & Medical Engineering

基　　金：国家自然科学基金项目(20505002)

摘　　要：目的研究雄性大鼠在模拟微重力下睾丸组织中类固醇激素合成相关基因表达的变化,探讨微重力对睾丸中睾酮合成的影响,以及这种影响与血浆睾酮含量变化的关系。方法建立无隐睾尾部悬吊大鼠的模拟微重力模型;采用酶联免疫竞争(ELISA)法测定大鼠血浆睾酮的含量;逆转录聚合酶链反应(RT-PCR)法检测睾丸组织中类固醇激素合成急性调节蛋白(StAR)、细胞色素P450-17α-羟化酶(P450c17)、3β-羟甾脱氢酶(3β-HSD)的mRNA水平的变化。结果雄性大鼠在模拟微重力条件下7d后,睾丸重量极显著减轻(P<0.01),血浆睾酮含量极显著下降(P<0.01),睾丸组织中StAR、P450c17、3β-HSD的mRNA水平显著下调(P<0.05)。结论模拟微重力引起雄性大鼠睾丸中StAR、P450c17、3β-HSD的表达下调,使睾酮合成途径受阻,导致睾酮分泌减少,进而造成血浆睾酮含量下降。Objective To study the mechanism of decreased secretion of testosterone in male rats under short-term simulated microgravity with examining mRNA of genes expression related to testosterone synthesis. Methods A tail-suspended.model of rat with its inguinal canal ligation to simulate microgravity was used. Testosterone level in blood plasma was measured with enzyme-linked immunosorbent assay （ELISA）. Reverse transcription-polymerase chain reaction （RT-PCR） was used to determine the expression of steroidogenic acute regulatory protein （STAR）, cytochrome P450- 17oL-hydroxylase （P450c17）, and 3β-hydroxysteroid dehydrogenase （3β-HSD） mRNAs in testis of rats. Results After 7 d tail-suspension, the weight of testis and the plasmatic testosterone level of tail-suspended rats both with or without inguinal canal ligation decreased significantly compared with controls （P 〈0.01 ）. The expression level of STAR, P450c17, 3β-HSD mRNAs in testis were significantly lower than those of the control group （P 〈 0.05）. Conclusion The expression of STAR, P450c17, 3β-HSD in testis decreases under short-term simulated microgravity, which leads to the decreased testosterone synthesis and secretion.

关 键 词：模拟微重力 睾丸 类固醇激素合成急性调节蛋白 细胞色素P450-17α-羟化酶 3β-羟甾脱氢酶 睾酮 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]