高脂血症和动脉粥样硬化不同痰瘀证候患者血浆蛋白质差异表达谱与脏腑功能的关系  

Relationship between plasma protein expression profiles and states of Zang-Fu organs in patients with phlegm or blood stagnation syndromes due to hyperlipidemia and atherosclerosis

作　　者：宋剑南[1] 刘军莲[1] 房祥忠[2] 胡元会[3] 雷燕[4] 牛晓红[1] 吴刚[5] 陈保生[5] 马雅銮[1] 陈冰[1] 金红[1] 

机构地区：[1]中国中医科学院中医基础理论研究所中医基础实验研究中心,北京100700 [2]北京大学数学科学学院概率统计系,北京100871 [3]中国中医科学院广安门医院心内科,北京100053 [4]中国中医科学院西苑医院心内科,北京100091 [5]中国医学科学院基础研究所生化教研室,北京100730

出　　处：《中西医结合学报》2008年第12期1233-1237,共5页Journal of Chinese Integrative Medicine

基　　金：国家自然科学基金重大计划重点资助项目(No.90209001);国家科技基础研究发展计划资助项目(No.2003CB517105)

摘　　要：目的:探讨高脂血症及动脉粥样硬化不同痰瘀证候患者血浆特异性蛋白质群与脏腑功能之间的相关性。方法:采用中医证候诊断,观察146例高脂血症及动脉粥样硬化不同痰瘀证候病人的脏腑功能状况。获取病人的血浆样品进行二维凝胶电泳,对差异表达蛋白进行二级质谱分析,寻找可区别不同痰瘀证候的特异性标志蛋白群。采用图模型分析方法研究不同痰瘀证候可能的标志蛋白与脏腑功能之间的相关性。结果:区分高脂血症及动脉粥样硬化病人中医痰证与痰瘀互阻证可能的标志蛋白群中纤维蛋白原γ链、白蛋白及载脂蛋白AI前体主要与心气虚、肾气虚及脾气虚有关;区分痰证与血瘀证可能的4种标志蛋白质中,白蛋白和肾上腺髓质素结合蛋白前体与心气虚、肾气虚均有关,结合珠蛋白前体只与肾气虚有关,并通过肾上腺髓质素结合蛋白前体与心气虚相关,补体C4与之均无关;区分血瘀证与痰瘀互阻证可能的标志蛋白群中白蛋白与心气虚、肾气虚及脾气虚均有关,而肾上腺髓质素结合蛋白前体只与脾气虚相关;区分非痰非瘀类与痰瘀类证候可能的标志蛋白纤维蛋白原β链主要与肾气虚有关,载脂蛋白AI前体主要与肾气虚及心气虚有关。结论:高脂血症及动脉粥样硬化不同痰瘀证候可能的血浆标志蛋白与不同的脏腑功能之间存在不同的关联组合。Objective. To investigate the relationship between the plasma biomarker proteins and the states of Zang-Fu organs in patients with phlegm or blood stagnation syndromes due to hyperlipidemia and atherosclerosis. Methods： The states of Zang-Fu organs in 146 patients with perlipidemia and atherosclerosis were diagnosed by syndrome differentiation of traditional Chinese medicine. The plasma proteins from these patients were separated by two-dimensional polyacrylamide gel electrophoresis （2-DE）. Differential protein profiling was established by Image Master 6.0 software, and the differential proteins were analyzed by quadrupole time of flight mass spectrometry （Q-TOF-MS）. The association between the plasma biomarker proteins and the states of Zang-Fu organs was analyzed by graphical models. Results： The biomarker proteins such as fibrinogen gamma chain, albumin and apolipoprotein AI （precursor） in discrimination of the patients with phlegm syndrome from phlegm accumulating with stagnation syndrome were correlated with the deficiency of kidney-qi, heart-qi and spteen-qi. Among the four biomarker proteins in discrimination of the patients with phlegm syndrome from blood stagnation syndrome, albumin, adrenomedullin binding protein （precursor） and haptoglobin （precursor） were correlated with the deficiency of kidney-qi and heart-qi, but complement component C4 was independent of the deficient Zang-Fu organs. The biomarker albumin was associated with the deficiency of kidney-qi, heart-qi and spleen-qi, and adrenomedullin binding protein （precursor） was correlated with the deficiency of spleen-qi in discrimination of the patients with blood stagnation syndrome from phlegm accumulating with stagnation syndrome. As the potential biomarker proteins in discrimination of the patients with non-phlegm and non-stagnation syndrome from phlegm accumulating with stagnation syndrome, the fibrinogen beta chain was related with the deficiency of kidney-qi, and apolipoprotein AI （precursor） was correlated w

关 键 词：高脂血症 动脉粥样硬化 痰证 血瘀 血浆蛋白质类 脏腑 

分 类 号：R259[医药卫生—中西医结合]